Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Phytochem Anal. 2018 Jan;29(1):112-117. doi: 10.1002/pca.2719. Epub 2017 Sep 12.
Tanshinones are a major class of bioactive ingredients in the traditional herbal medicines, Danshen (Salvia miltiorrhiza). A sensitive and reliable determination method for tanshinones is useful to ensure the quality of Danshen.
To develop a sensitive and selective analytical method for tanshinones by high-performance liquid chromatography (HPLC) with fluorescence detection after pre-column derivatisation.
The proposed method depends on derivatisation reaction of tanshinones with 4-carbomethoxybenzaldehyde and ammonium acetate forming intensely fluorescent imidazole derivative.
The proposed method provided excellent sensitivity with the detection limits of 3.3 nM (66 fmol/injection), 3.2 nM (64 fmol/injection) and 2.0 nM (40 fmol/injection) for cryptotanshinone, tanshinone I and tanshinone IIA, respectively, without the necessity of complicated instrumentations. The developed method is successfully applied to quantify the contents of tanshinones in Danshen.
The developed method is the first analytical method for tanshinones by fluorescence detection. Since the derivatisation reaction is selective for the o-quinone structure of tanshinone, the developed method will become a suitable mean for the discovering of tanshinone type diterpenoids from herbal samples. Copyright © 2017 John Wiley & Sons, Ltd.
丹参酮是传统草药丹参（Salvia miltiorrhiza）中的一类主要生物活性成分。丹参酮的灵敏可靠的测定方法有助于确保丹参的质量。
建立一种灵敏、选择性的丹参酮分析方法，采用高效液相色谱（HPLC）-荧光检测法，柱前衍生化。
该方法依赖于丹参酮与 4-甲氧基苯甲醛和醋酸铵的衍生化反应，形成强荧光咪唑衍生物。
该方法提供了极好的灵敏度，隐丹参酮、丹参酮 I 和丹参酮 IIA 的检测限分别为 3.3 nM（66 fmol/进样）、3.2 nM（64 fmol/进样）和 2.0 nM（40 fmol/进样），无需复杂的仪器。该方法成功应用于定量测定丹参中的丹参酮含量。
该方法是丹参酮荧光检测的第一种分析方法。由于衍生化反应对丹参酮的邻醌结构具有选择性，因此该方法将成为从草药样品中发现丹参酮型二萜类化合物的合适手段。版权所有©2017 约翰威立父子公司
