高效液相色谱-质谱法测定益母草制剂中盐酸水苏碱和盐酸益母草碱
[Determination of stachydrine hydrochloride and leonurine hydrochloride in Yimucao preparations by HPLC-MS].
作者信息
Zhou Chao, He Yi, Zhang Yu-Mei, Wang Bo, Yu Jian-Dong, Dai Zhong, Ma Shuang-Cheng
机构信息
Qiqihaer Food and Drug Inspection Control, Qiqihaer 161000, China.
National Institutes for Food and Drug Control, Beijing 100050, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2016 May;41(10):1876-1879. doi: 10.4268/cjcmm20161019.
To establish the quantitative method of stachydrine hydrochloride and leonurine hydrochloride in the preparations of Leonuri Herba. The contents of stachydrine hydrochloride and leonurine hydrochloride in the preparations of Leonuri Herba were determined by HPLC-MS. The chromatographic column was Waters XBridge Amide(4.6 mm×250 mm,5 μm). The mobile phase was acetonitrile-0.1% formic acid in gradient mode,at the flow rate of 1.0 mL• min⁻¹,with the split ratio of 1∶4. MS conditions for the ESI ion source,positive ion mode,selective ion scan(SIM) of stachydrine hydrochloride(m/z 144.0) and leonurine hydrochloride(m/z 312.0) was measured. The linear ranges of stachydrine hydrochloride was 0.562 8-281.4 μg•L-1（r=0.999 8）. The linear ranges of leonurine hydrochloride was 0.521 2-260.6 μg•L-1（r=0.999 8）. The method is accurate,simple,and reliable,and can be used to determine the contents of stachydrine hydrochloride and leonurine hydrochloride in the preparations of Leonuri Herba.
建立益母草制剂中盐酸水苏碱和盐酸益母草碱的定量测定方法。采用高效液相色谱 - 质谱联用法测定益母草制剂中盐酸水苏碱和盐酸益母草碱的含量。色谱柱为Waters XBridge Amide（4.6 mm×250 mm，5 μm）。流动相为乙腈 - 0.1%甲酸，采用梯度洗脱模式，流速为1.0 mL•min⁻¹，分流比为1∶4。质谱条件为电喷雾离子源（ESI），正离子模式，对盐酸水苏碱（m/z 144.0）和盐酸益母草碱（m/z 312.0）进行选择性离子扫描（SIM）测定。盐酸水苏碱的线性范围为0.562 8 - 281.4 μg•L⁻¹（r = 0.999 8）。盐酸益母草碱的线性范围为0.521 2 - 260.6 μg•L⁻¹（r = 0.999 8）。该方法准确、简便、可靠，可用于测定益母草制剂中盐酸水苏碱和盐酸益母草碱的含量。
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