Zhao Yu-Kun, Li Li, Liu Xue, Cheng Shi-Ping, Guo Qing-Qing, Fan Dan-Ping, Zheng Kang, He Xiao-Juan, Lv Ai-Ping
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2016 May;41(10):1916-1920. doi: 10.4268/cjcmm20161026.
To explore the pharmacological mechanism of glycyrrhizin with series methods of systems pharmacology, main diseases related to glycyrrhizin were obtained by text mining tool; and the target proteins of glycyrrhizin were obtained via the database of Polysearch and PubChem. Then, the target proteins interaction network of glycyrrhizin was built using the software called Cytoscape. Next, the protein groups related to glycyrrhizin were analyzed by using Gene Ontology (GO) tool, and the action pathway of its target proteins was analyzed by using enrichment method. Text mining results showed that the related diseases of glycyrrhizin included chronic hepatitis C, chronic hepatitis, hepatitis, HIV virus, liver cancer and so on. Gene ontology analysis indicated that glycyrrhizin played a role mainly through modification of proteins and chromatin. The signaling pathway enrichment results showed that the main action proteins of glycyrrhizin were related to MAPK signaling pathway, toll-like receptor signaling pathway, neurotrophic factor signaling pathway, cancer and apoptosis pathways. So we can conclude that glycyrrhizin may exert its biological functions primarily by regulating multiple pathways such as MAPK signaling pathway and Toll-like receptors signaling pathway. The pharmacological action of a drug can be rapidly and comprehensively analyzed by the ways of systems pharmacology.
采用系统药理学的系列方法探索甘草酸的药理机制，通过文本挖掘工具获取与甘草酸相关的主要疾病；并通过Polysearch数据库和PubChem获取甘草酸的靶蛋白。然后，使用Cytoscape软件构建甘草酸的靶蛋白相互作用网络。接下来，利用基因本体论（GO）工具分析与甘草酸相关的蛋白组，并采用富集方法分析其靶蛋白的作用途径。文本挖掘结果表明，甘草酸的相关疾病包括丙型慢性肝炎、慢性肝炎、肝炎、HIV病毒、肝癌等。基因本体分析表明，甘草酸主要通过修饰蛋白质和染色质发挥作用。信号通路富集结果表明，甘草酸的主要作用蛋白与MAPK信号通路、Toll样受体信号通路、神经营养因子信号通路、癌症和凋亡通路有关。因此可以得出结论，甘草酸可能主要通过调节MAPK信号通路和Toll样受体信号通路等多种途径发挥其生物学功能。通过系统药理学的方法可以快速、全面地分析药物的药理作用。
