Huang Lei, Zhao Tingting, He Yun, Liu Yilin, Liu Chang, Jin Dayao, Jia Xiaoqiang
Key Laboratory of Systems Bioengineering (Ministry of Education), Department of Biological Engineering School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Sheng Wu Gong Cheng Xue Bao. 2017 Jun 25;33(6):957-967. doi: 10.13345/j.cjb.160472.
We studied the remediation of petroleum-contaminated soils. Two Pseudomonas aeruginosa strains were screened from soil samples in the mine environment. The degradation rate of crude oil was 95.67% after 10 days by mixed culture of the two strains, which was 32% higher than that of single bacterium. It means that the two bacteria had synergistic effect on degrading crude oil. Based on the result, bacterial agent was prepared, and the oil pollution sites were artificially constructed to simulate the degradation of crude oil under different operating conditions. During the experiment, the petroleum hydrocarbon content of the sites after treating with the bacterial agent, decreased obviously in 60 days. The content decreased to among 0.1% and 0.3% from the initial 0.8% per gram of soil. Then, the site with organic manure as supplemental source of carbon and nitrogen had the highest degradation rate of 85.28%, compared to that without adding bacterial agent of only 25.85%.
我们研究了石油污染土壤的修复。从矿山环境的土壤样本中筛选出两株铜绿假单胞菌。两株菌混合培养10天后，原油降解率为95.67%，比单菌培养高出32%。这意味着这两种细菌在降解原油方面具有协同作用。基于该结果，制备了菌剂，并人工构建油污场地以模拟不同操作条件下原油的降解。实验期间，经菌剂处理后的场地石油烃含量在60天内明显下降。含量从每克土壤最初的0.8%降至0.1%至0.3%之间。然后，以有机肥作为碳氮补充源的场地降解率最高，为85.28%，而不添加菌剂的场地降解率仅为25.85%。
