von Dorsche H H, Reiher H, Hahn H J, Fält K, Falkmer S
Institute of Anatomy, Ernst-Moritz-Arndt-University, Greifswald, GDR.
Diabetes Res. 1987 Aug;5(4):157-61.
Tissue culture of human foetal pancreas slices, obtained at a gestational age between 10 and 19 weeks, as a method of preservation and to pool the material before transplantation was investigated. Before and after 2 weeks of culture the pancreatic insulin content, the insulin secretion in response to glucose and isobutylmethylxanthin (IBMX) as well as the protein biosynthesis were measured. In addition, the distribution of the insulin immunoreactive cells was examined, as well as their relative volume density. After 2 weeks' culture an increase of the basal insulin secretion was observed; this was probably due to the glucose content (10 mmol/l) in the culture medium. Neither the stimulated insulin secretion, nor the protein biosynthesis, the insulin content, and the B-cell volume were altered by the used culture conditions. It was concluded that tissue culture is a suitable method to preserve human foetal pancreas slices before transplantation.
研究了获取孕龄在10至19周之间的人胎儿胰腺切片进行组织培养，作为一种保存方法以及在移植前汇集材料的方式。在培养2周前后，测量了胰腺胰岛素含量、对葡萄糖和异丁基甲基黄嘌呤（IBMX）的胰岛素分泌以及蛋白质生物合成。此外，检查了胰岛素免疫反应性细胞的分布及其相对体积密度。培养2周后观察到基础胰岛素分泌增加；这可能归因于培养基中的葡萄糖含量（10 mmol/l）。所用培养条件未改变刺激后的胰岛素分泌、蛋白质生物合成、胰岛素含量和B细胞体积。得出的结论是，组织培养是在移植前保存人胎儿胰腺切片的合适方法。
