从日本鹌鹑（Coturnix coturnix japonica）中分离并鉴定单个心肌细胞。

Isolation and characterization of single myocardial cells from the quail, Coturnix coturnix japonica.

Karttunen P, Tirri R

Department of Biology, University of Turku, Finland.

Comp Biochem Physiol A Comp Physiol. 1987;88(1):161-6. doi: 10.1016/0300-9629(87)90115-0.

Abstract

The enzymatic cell isolation technique was applied to the bird heart resulting in myocytes of which 10-50% maintained their spindle-shaped morphology, excluded the vital dye, Evans blue and tolerated physiological concentration of Ca2+ ions. 2. The length of spindle-shaped myocytes was on average 289 +/- 7 microns, and the maximum width was 10.2 +/- 0.3 microns. The mean length of the sarcomeres was 2.18 +/- 0.03 microns. 3. In electron micrographs the fine structure of the spindle-shaped myocytes looked normal--regular sarcomeric organization with clear A and I bands, mitochondria with tightly located cristae and well-developed sarcoplasmic reticulum (SR). 4. Most (80%) of the spindle-shaped myocytes were quiescent in physiological calcium concentration and practically all of them could be induced to twitch by electric field stimulation. Some beat spontaneously showing mostly slowly-propagating (135 +/- 6 microns/sec at 20 degrees C) contraction waves, so-called phasic contractions. Sometimes spontaneous twitch-type contractions could also be seen.

摘要

酶促细胞分离技术应用于鸟类心脏，得到的心肌细胞中，10%至50%保持其纺锤形形态，排斥活体染料伊文思蓝，并耐受生理浓度的钙离子。2. 纺锤形心肌细胞的平均长度为289±7微米，最大宽度为10.2±0.3微米。肌节的平均长度为2.18±0.03微米。3. 在电子显微镜照片中，纺锤形心肌细胞的精细结构看起来正常——肌节组织规则，A带和I带清晰，线粒体嵴紧密排列，肌浆网（SR）发达。4. 大多数（80%）纺锤形心肌细胞在生理钙浓度下静止，实际上所有细胞都可通过电场刺激诱导抽搐。一些细胞自发搏动，主要表现为传播缓慢的（20℃时为135±6微米/秒）收缩波，即所谓的阶段性收缩。有时也可见自发的抽搐型收缩。