Tate J M, McDonnell T J, Oberpriller J C, Oberpriller J O
Tissue Cell. 1987;19(4):577-85. doi: 10.1016/0040-8166(87)90049-8.
This report describes a technique which permits a high yield of viable adult cardiac myocytes from the adult newt using enzymatic separation techniques at low temperature and high enzyme concentrations. Observations by light microscopy showed the isolated myocytes to have a distinctively slender morphology which consisted of a variable number of arm-like appendages radiating from the center of cells which were predominantly mononucleated. Atrial myocytes were typically observed to have two to three arm-like appendages while ventricular myocytes typically had three to six appendages. The majority of myocytes displayed normal fine structure when examined by transmission electron microscopy. Computerized image analysis revealed that atrial cells were significantly greater in cell length (192.9 +/- 53.4 microns) and in nuclear length (25 +/- 5.3 microns) and perimeter (59.2 +/- 10.7 microns) than were ventricular cells (162.8 +/- 39 microns, 23.6 +/- 5.1 microns and 57.4 +/- 11.1 microns, respectively), while cell widths and areas were greater in ventricular cells (16.5 +/- 4.7 microns and 1839.8 +/- 585.0 microns, respectively) than in atrial cells (13.2 +/- 3.1 microns and 1520.3 +/- 527.6 microns, respectively). Comparison of these data with previous descriptions of isolated amphibian and mammalian cardiac myocytes emphasizes species-related differences.
本报告描述了一种技术，该技术可在低温和高酶浓度条件下，使用酶分离技术从成年蝾螈中获得高产量的存活成年心肌细胞。光学显微镜观察显示，分离出的心肌细胞具有独特的细长形态，由从细胞中心辐射出的数量可变的臂状附属物组成，这些细胞主要为单核。通常观察到心房肌细胞有两到三个臂状附属物，而心室肌细胞通常有三到六个附属物。透射电子显微镜检查显示，大多数心肌细胞显示出正常的精细结构。计算机图像分析显示，心房细胞的细胞长度（192.9±53.4微米）、核长度（25±5.3微米）和周长（59.2±10.7微米）显著大于心室细胞（分别为162.8±39微米、23.6±5.1微米和57.4±11.1微米），而心室细胞的细胞宽度和面积（分别为16.5±4.7微米和1839.8±585.0微米）大于心房细胞（分别为13.2±3.1微米和1520.3±527.6微米）。将这些数据与先前对分离的两栖动物和哺乳动物心肌细胞的描述进行比较，突出了物种相关的差异。
