Research and Technology Directorate, US Army Edgewood Chemical Biological Center (ECBC) , Aberdeen Proving Ground, Maryland 21010, United States.
Excet, Inc. , 6225 Brandon Ave, Suite 360, Springfield, Virginia 22150, United States.
Anal Chem. 2017 Oct 17;89(20):10866-10872. doi: 10.1021/acs.analchem.7b02530. Epub 2017 Sep 25.
Paper spray ionization mass spectrometry offers a rapid alternative platform requiring no sample preparation. Aerosolized chemical warfare agent (CWA) simulants trimethyl phosphate, dimethyl methylphosphonate, and diisopropyl methylphosphonate were captured by passing air through a glass fiber filter disk within a disposable paper spray cartridge. CWA simulants were aerosolized at varying concentrations using an in-house built aerosol chamber. A custom 3D-printed holder was designed and built to facilitate the aerosol capture onto the paper spray cartridges. The air flow through each of the collection devices was maintained equally to ensure the same volume of air sampled across methods. Each approach yielded linear calibration curves with R values between 0.98-0.99 for each compound and similar limits of detection in terms of disbursed aerosol concentration. While the glass fiber filter disk has a higher capture efficiency (≈40%), the paper spray method produces analogous results even with a lower capture efficiency (≈1%). Improvements were made to include glass fiber filters as the substrate within the paper spray cartridge consumable. Glass fiber filters were then treated with ammonium sulfate to decrease chemical interaction with the simulants. This allowed for improved direct aerosol capture efficiency (>40%). Ultimately, the limits of detection were reduced to levels comparable to current worker population limits of 1 × 10 mg/m.
纸喷雾电离质谱提供了一种快速的替代平台，无需样品制备。气溶胶化的化学战剂（CWA）模拟物三甲基磷酸酯、二甲甲基膦酸酯和二异丙基甲基膦酸酯通过在一次性纸喷雾管内的玻璃纤维滤盘过滤空气而被捕获。使用内部建造的气溶胶室以不同浓度气溶胶化 CWA 模拟物。设计并制造了定制的 3D 打印支架，以方便将气溶胶捕获到纸喷雾管上。通过每个收集装置的空气流量保持相等，以确保在方法之间采集相同体积的空气。每种方法都产生了线性校准曲线，每个化合物的 R 值在 0.98-0.99 之间，并且在分散气溶胶浓度方面具有相似的检测限。虽然玻璃纤维滤盘的捕获效率较高（≈40%），但即使在较低的捕获效率（≈1%）下，纸喷雾方法也能产生类似的结果。进行了改进，将玻璃纤维过滤器作为纸喷雾管消耗品内的基底包含在内。然后，用硫酸铵处理玻璃纤维过滤器以减少与模拟物的化学相互作用。这允许提高直接气溶胶捕获效率（>40%）。最终，检测限降低到与当前工人人群限制 1 × 10 mg/m 相当的水平。
