Lu Chen D, Lee ByungKun, Schottenhamml Julia, Maier Andreas, Pugh Edward N, Fujimoto James G
Research Laboratory of Electronics and Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States.
Pattern Recognition Laboratory, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany.
Invest Ophthalmol Vis Sci. 2017 Sep 1;58(11):4632-4643. doi: 10.1167/iovs.17-22171.
To examine outer retinal band changes after flash stimulus and subsequent dark adaptation with ultrahigh-resolution optical coherence tomography (UHR-OCT).
Five dark-adapted left eyes of five normal subjects were imaged with 3-μm axial-resolution UHR-OCT during 30 minutes of dark adaptation following 96%, 54%, 23%, and 0% full-field and 54% half-field rhodopsin bleach. We identified the ellipsoid zone inner segment/outer segment (EZ[IS/OS]), cone interdigitation zone (CIZ), rod interdigitation zone (RIZ), retinal pigment epithelium (RPE), and Bruch's membrane (BM) axial positions and generated two-dimensional thickness maps of the EZ(IS/OS) to the four bands. The average thickness over an area of the thickness map was compared against that of the dark-adapted baselines. The time-dependent thickness changes (photoresponses) were statistically compared against 0% bleach. Dark adaptometry was performed with the same bleaching protocol.
The EZ(IS/OS)-CIZ photoresponse was significantly different at 96% (P < 0.0001) and 54% (P = 0.006) bleach. At all three bleaching levels, the EZ(IS/OS)-RIZ, -RPE, and -BM responses were significantly different (P < 0.0001). The EZ(IS/OS)-CIZ and EZ(IS/OS)-RIZ time courses were similar to the recovery of rod- and cone-mediated sensitivity, respectively, measured with dark adaptometry. The maximal EZ(IS/OS)-CIZ and EZ(IS/OS)-RIZ response magnitudes doubled from 54% to 96% bleach. Both EZ(IS/OS)-RPE and EZ(IS/OS)-BM responses resembled dampened oscillations that were graded in amplitude and duration with bleaching intensity. Half-field photoresponses were localized to the stimulated retina.
With noninvasive, near-infrared UHR-OCT, we characterized three distinct, spatially localized photoresponses in the outer retinal bands. These photoresponses have potential value as physical correlates of photoreceptor function.
使用超高分辨率光学相干断层扫描（UHR-OCT）检查闪光刺激及随后的暗适应后视网膜外层带的变化。
对5名正常受试者的5只暗适应左眼，在96%、54%、23%和0%全视野以及54%半视野视紫红质漂白后的30分钟暗适应过程中，用轴向分辨率为3μm的UHR-OCT进行成像。我们确定了椭圆体带内节/外节（EZ[IS/OS]）、锥体细胞指状交叉带（CIZ）、杆体细胞指状交叉带（RIZ）、视网膜色素上皮（RPE）和布鲁赫膜（BM）的轴向位置，并生成了EZ(IS/OS)到这四条带的二维厚度图。将厚度图区域内的平均厚度与暗适应基线的平均厚度进行比较。将随时间变化的厚度变化（光反应）与0%漂白的情况进行统计学比较。使用相同的漂白方案进行暗适应测量。
在96%（P < 0.0001）和54%（P = 0.006）漂白时，EZ(IS/OS)-CIZ光反应有显著差异。在所有三个漂白水平下，EZ(IS/OS)-RIZ、-RPE和-BM反应均有显著差异（P < 0.0001）。EZ(IS/OS)-CIZ和EZ(IS/OS)-RIZ的时间进程分别与用暗适应测量法测得的杆体和锥体介导的敏感度恢复情况相似。最大的EZ(IS/OS)-CIZ和EZ(IS/OS)-RIZ反应幅度从54%漂白增加到96%漂白时翻倍。EZ(IS/OS)-RPE和EZ(IS/OS)-BM反应均类似于衰减振荡，其幅度和持续时间随漂白强度而分级。半视野光反应局限于受刺激的视网膜。
通过无创的近红外UHR-OCT，我们在视网膜外层带中表征了三种不同的、空间定位的光反应。这些光反应作为光感受器功能的物理相关指标具有潜在价值。
