BK21 Program, Department of Oral Biochemistry and Institute of Oral Bioscience, School of Dentistry, Chonbuk National University, Jeonju, Korea.
Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Eumsung, Korea.
Int Endod J. 2018 Apr;51(4):438-447. doi: 10.1111/iej.12861. Epub 2017 Oct 7.
To examine the properties of Schisandrin C as an anti-inflammatory and antioxidant compound, and whether its characteristics promote mitochondrial biogenesis in human dental pulp cells (HDPCs).
HDPCs were extracted from fresh third molars and cultured for experiments. Reactive oxidative stress (ROS) and nitric oxide (NO) formation were analysed by a Muse cell analyser. Western blotting and gelatin zymography were used to identify the presence of antioxidants, as well as anti-inflammatory and mitochondrial biogenesis with specific antibody. An unpaired Student's t-test was used for statistical analysis.
Schisandrin C inhibited lipopolysaccharide-stimulated inflammatory molecules; interleukin 1 beta, tumour necrosis factor alpha, intracellular adhesion molecule-1, vascular cell adhesion molecule-1, matrix metalloproteinase-2 and -9, NO production, ROS formation, nuclear factor kappa B translocation (P < 0.05) through the mitogen-activated protein kinase pathway. Schisandrin C increased the expression of superoxide dismutase enzymes as well as haem oxygenase-1 and peroxisome proliferator-activated receptor gamma coactivator 1-alpha through the phosphorylated-protein kinase B (p-Akt) and nuclear factor erythroid 2-related factor-2 pathways (P < 0.05). The anti-inflammatory and antioxidant properties of Schisandrin C promoted mitochondrial biogenesis.
Schisandrin C has the potential to reduce inflammation and oxidation and to promote mitochondrial biogenesis. Therefore, Schisandrin C may be considered for use as an anti-inflammatory compound for oral inflammation through mitochondrial biogenesis.
研究五味子丙素作为一种抗炎和抗氧化化合物的特性，以及其特性是否促进人牙髓细胞（HDPCs）中的线粒体生物发生。
从新鲜的第三磨牙中提取 HDPCs 并进行实验培养。通过 Muse 细胞分析仪分析活性氧化应激（ROS）和一氧化氮（NO）的形成。使用 Western blot 和明胶酶谱分析鉴定抗氧化剂的存在，以及抗炎和线粒体生物发生与特异性抗体。使用未配对的学生 t 检验进行统计分析。
五味子丙素通过丝裂原活化蛋白激酶途径抑制脂多糖刺激的炎症分子；白细胞介素 1β、肿瘤坏死因子-α、细胞间黏附分子-1、血管细胞黏附分子-1、基质金属蛋白酶-2 和 -9、NO 产生、ROS 形成、核因子 kappa B 易位（P<0.05）。五味子丙素通过磷酸化蛋白激酶 B（p-Akt）和核因子红系 2 相关因子-2 途径增加超氧化物歧化酶酶以及血红素加氧酶-1 和过氧化物酶体增殖物激活受体γ共激活因子 1-α的表达（P<0.05）。五味子丙素的抗炎和抗氧化特性促进了线粒体生物发生。
五味子丙素具有减轻炎症和氧化作用并促进线粒体生物发生的潜力。因此，五味子丙素可通过线粒体生物发生被认为是用于口腔炎症的抗炎化合物。
