Celis J E, Madsen P, Lauridsen J B
Department of Medical Biochemistry, Aarhus University, Denmark.
Leukemia. 1987 Jul;1(7):568-71.
Indirect immunofluorescence staining of synchronized lymphoid human Molt-4 cells with proliferating cell nuclear antigen autoantibodies specific for cyclin revealed nucleolar staining only in cells in mid to late S-phase. These results together with similar earlier observations in epithelial and fibroblasts cells indicate that this organelle replicates in mid to late S-phase in cultured somatic cells.
用细胞周期蛋白特异性增殖细胞核抗原自身抗体对同步化的人淋巴样Molt-4细胞进行间接免疫荧光染色，结果显示仅在S期中期至后期的细胞中出现核仁染色。这些结果与早期在上皮细胞和成纤维细胞中进行的类似观察结果共同表明，在培养的体细胞中，这种细胞器在S期中期至后期进行复制。
