增殖细胞核抗原（细胞周期蛋白）自身抗体和单克隆抗体在人类培养细胞中显示出相似的细胞周期蛋白（增殖细胞核抗原）抗原染色模式。
PCNA (cyclin) autoantibodies and monoclonal antibodies reveal similar patterns of cyclin (PCNA) antigen staining in human cultured cells.
作者信息
Madsen P, Ogata K, Celis J E
机构信息
Department of Medical Biochemistry, Aarhus University, Denmark.
出版信息
Leukemia. 1987 Mar;1(3):220-5.
Double immunofluorescence and [3H]thymidine autoradiographic analysis of the same field of transformed human amnion cells (AMA) reacted with proliferating cell nuclear antigen (PCNA) autoantibodies and a monoclonal antibody (mAb 19F4) specific for cyclin (PCNA) revealed similar patterns and sequence of cyclin (PCNA) antigen staining during S-phase. These results suggest that immunofluorescence patterns obtained with PCNA autoantibodies reflect in fact patterns of cyclin (PCNA) antigen staining.
对转化的人羊膜细胞（AMA）的同一视野进行双重免疫荧光和[3H]胸腺嘧啶核苷放射自显影分析，该细胞与增殖细胞核抗原（PCNA）自身抗体以及细胞周期蛋白（PCNA）特异性单克隆抗体（mAb 19F4）反应，结果显示在S期细胞周期蛋白（PCNA）抗原染色具有相似的模式和序列。这些结果表明，用PCNA自身抗体获得的免疫荧光模式实际上反映了细胞周期蛋白（PCNA）抗原染色的模式。
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