Madsen P, Ogata K, Celis J E
Department of Medical Biochemistry, Aarhus University, Denmark.
Leukemia. 1987 Mar;1(3):220-5.
Double immunofluorescence and [3H]thymidine autoradiographic analysis of the same field of transformed human amnion cells (AMA) reacted with proliferating cell nuclear antigen (PCNA) autoantibodies and a monoclonal antibody (mAb 19F4) specific for cyclin (PCNA) revealed similar patterns and sequence of cyclin (PCNA) antigen staining during S-phase. These results suggest that immunofluorescence patterns obtained with PCNA autoantibodies reflect in fact patterns of cyclin (PCNA) antigen staining.
对转化的人羊膜细胞（AMA）的同一视野进行双重免疫荧光和[3H]胸腺嘧啶核苷放射自显影分析，该细胞与增殖细胞核抗原（PCNA）自身抗体以及细胞周期蛋白（PCNA）特异性单克隆抗体（mAb 19F4）反应，结果显示在S期细胞周期蛋白（PCNA）抗原染色具有相似的模式和序列。这些结果表明，用PCNA自身抗体获得的免疫荧光模式实际上反映了细胞周期蛋白（PCNA）抗原染色的模式。
