三氟乙酸水解后酸橙（Citrus aurantifolia）和葡萄柚（Citrus paradisi）角质的比较研究。

Comparative studies of cutins from lime (Citrus aurantifolia) and grapefruit (Citrus paradisi) after TFA hydrolysis.

作者信息

Hernández Velasco Brenda Liliana, Arrieta-Baez Daniel, Cortez Sotelo Pedro Iván, Méndez-Méndez Juan Vicente, Berdeja Martínez Blanca Margarita, Gómez-Patiño Mayra Beatriz

机构信息

Instituto Politécnico Nacional - ENCB, Av. Wilfrido Massieu, Unidad Adolfo López Mateos, 07738, Ciudad de México, CDMX, Mexico.

Instituto Politécnico Nacional - CNMN, Av. Luis Enrique Erro s/n, Nueva Industrial Vallejo, 07738, Ciudad de México, CDMX, Mexico.

出版信息

Phytochemistry. 2017 Dec;144:78-86. doi: 10.1016/j.phytochem.2017.08.017. Epub 2017 Sep 9.

DOI:10.1016/j.phytochem.2017.08.017

PMID:28898741

Abstract

Grapefruit and lime cutins were analyzed and compared in order to obtain information about their cutin architecture. This was performed using a sequential hydrolysis, first with trifluoroacetic acid to remove most of the polysaccharides present in the cutins, followed by an alkaline hydrolysis in order to obtain the main aliphatic compounds. Analysis by CPMAS C NMR and ATR FT-IR of the cutins after 2.0 M TFA revealed that grapefruit cutin has independent aliphatic and polysaccharide domains while in the lime cutin these components could be homogeneously distributed. These observations were in agreement with an AFM analysis of the cutins obtained in the hydrolysis reactions. The main aliphatic compounds were detected and characterized as 16-hydroxy-10-oxo-hexadecanoic acid and 10,16-dihydroxyhexadecanoic acid. These were present in grapefruit cutin at 35.80% and 21.86% and in lime cutin at 20.44% and 40.36% respectively.

摘要

为了获取有关葡萄柚和酸橙角质层结构的信息，对它们的角质层进行了分析和比较。这是通过顺序水解来完成的，首先用三氟乙酸去除角质层中存在的大部分多糖，然后进行碱性水解以获得主要的脂肪族化合物。对经过2.0 M三氟乙酸处理后的角质层进行交叉极化魔角旋转碳核磁共振（CPMAS C NMR）和衰减全反射傅里叶变换红外光谱（ATR FT-IR）分析表明，葡萄柚角质层具有独立的脂肪族和多糖结构域，而在酸橙角质层中，这些成分可能均匀分布。这些观察结果与水解反应中获得的角质层的原子力显微镜（AFM）分析结果一致。检测到主要的脂肪族化合物并将其表征为16-羟基-10-氧代十六烷酸和10,16-二羟基十六烷酸。它们在葡萄柚角质层中的含量分别为35.80%和21.86%，在酸橙角质层中的含量分别为20.44%和40.36%。