用于同时检测鼻拭子中耐甲氧西林金黄色葡萄球菌和甲氧西林敏感金黄色葡萄球菌的 cobas MRSA/SA 检测法的性能
Performance of the cobas MRSA/SA Test for Simultaneous Detection of Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus From Nasal Swabs.
作者信息
Peterson Lance R, Woods Christopher W, Davis Thomas E, Wang Zi-Xuam, Young Stephen A, Osiecki John C, Lewinski Michael A, Liesenfeld Oliver
机构信息
Department of Laboratory Medicine and Pathology, NorthShore University HealthSystem, Evanston, IL.
Duke University Health System, Durham, NC.
出版信息
Am J Clin Pathol. 2017 Aug 1;148(2):119-127. doi: 10.1093/ajcp/aqx040.
OBJECTIVES
Health care-associated methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus aureus (SA) infections are continuing problems. Rapidly determining the MRSA colonization status of a patient facilitates practice to reduce spread of MRSA clinical disease. Sensitive detection of all SA prior to surgery, followed by decolonization, can significantly reduce postoperative infection from this pathogen. Our goal was to validate a new automated assay for this testing.
METHODS
We compared performance of the cobas MRSA/SA Test on the cobas 4800 System to direct and enriched chromogenic culture using nasal swabs collected from patients at six United States sites.
RESULTS
Compared to direct and enriched culture, the sensitivity for MRSA and SA was 93.1% and 93.9%, and the specificity was 97.5% and 94.2%, respectively. After discrepancy analysis, the sensitivity for MRSA and SA was 97.1% and 98.6%, and the specificity was 98.3% and 95.5%, respectively. Compared to direct culture, sensitivity for detecting any SA was 99.6%.
CONCLUSIONS
The cobas MRSA/SA Test is an effective tool to simultaneously perform surveillance testing for nasal colonization of both MRSA and MSSA.
目的
医疗保健相关的耐甲氧西林金黄色葡萄球菌（MRSA）和金黄色葡萄球菌（SA）感染一直是问题。快速确定患者的MRSA定植状态有助于采取措施减少MRSA临床疾病的传播。术前灵敏检测所有SA，随后进行去定植，可显著降低该病原体引起的术后感染。我们的目标是验证一种用于此项检测的新型自动化检测方法。
方法
我们将cobas 4800系统上的cobas MRSA/SA检测与使用从美国六个地点的患者采集的鼻拭子进行的直接和富集显色培养的性能进行了比较。
结果
与直接和富集培养相比，MRSA和SA的灵敏度分别为93.1%和93.9%，特异性分别为97.5%和94.2%。经过差异分析后，MRSA和SA的灵敏度分别为97.1%和98.6%，特异性分别为98.3%和95.5%。与直接培养相比，检测任何SA的灵敏度为99.6%。
结论
cobas MRSA/SA检测是同时对MRSA和MSSA鼻定植进行监测检测的有效工具。
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