Wu Cheng-Jun, Wang Zhao-Yan, Yang Yin-Xiang, Luan Zuo
Third Clinical Medical College, Southern Medical University, Guangzhou 510515, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2017 Sep;19(9):1003-1007. doi: 10.7499/j.issn.1008-8830.2017.09.014.
To investigate the long-term effect of oligodendrocyte precursor cell (OPC) transplantation on a rat model of white matter injury (WMI) in the preterm infant.
A total of 80 Sprague-Dawley rats aged 3 days were randomly divided into sham-operation group, model control group, 5-day ventricular/white matter transplantation group, 9-day ventricular/white matter transplantation group, 14-day ventricular/white matter transplantation group (n=10 each). All groups except the sham-operation group were treated with right common carotid artery ligation and hypoxia for 80 minutes to establish a rat model of WMI in the preterm infant. OPCs were prepared from the human fetal brain tissue (10-12 gestational weeks). At 5, 9, and 14 days after modeling, 3×10 OPCs were injected into the right lateral ventricle or white matter in each transplantation group, and myelin sheath and neurological function were evaluated under an electron microscope at ages of 60 and 90 days.
Electron microscopy showed that at an age of 60 days, each transplantation group had a slight improvement in myelin sheath injury compared with the model control group; at an age of 90 days, each transplantation group had significantly thickened myelin sheath and reduced structural damage compared with the model control group, and the 14-day transplantation groups had the most significant changes. There were no significant differences in the degree of myelin sheath injury between the ventricular and white matter transplantation groups at different time points. At an age of 60 or 90 days, the transplantation groups had a significantly higher modified neurological severity score (mNSS) than the sham-operation group and a significantly lower mNSS than the model control group (P<0.05).
OPC transplantation may have a long-term effect in the treatment of WMI in the preterm infant, and delayed transplantation may enhance its therapeutic effect.
探讨少突胶质前体细胞（OPC）移植对早产婴儿脑白质损伤（WMI）大鼠模型的长期影响。
将80只3日龄的Sprague-Dawley大鼠随机分为假手术组、模型对照组、5日龄脑室/白质移植组、9日龄脑室/白质移植组、14日龄脑室/白质移植组（每组n = 10）。除假手术组外，其余各组均采用右侧颈总动脉结扎并缺氧80分钟的方法建立早产婴儿WMI大鼠模型。OPC取自人胎儿脑组织（妊娠10 - 12周）。建模后5天、9天和14天，各移植组将3×10个OPC注入右侧脑室或白质，在60日龄和90日龄时通过电子显微镜评估髓鞘和神经功能。
电子显微镜检查显示，60日龄时，各移植组髓鞘损伤较模型对照组略有改善；90日龄时，各移植组髓鞘明显增厚，结构损伤较模型对照组减轻，其中14天移植组变化最为显著。不同时间点脑室和白质移植组之间髓鞘损伤程度无显著差异。在60日龄或90日龄时，移植组改良神经功能缺损评分（mNSS）显著高于假手术组，显著低于模型对照组（P<0.05）。
OPC移植可能对早产婴儿WMI的治疗具有长期效果，延迟移植可能增强其治疗效果。
