Department of Biology, Western Kentucky University, 1906 College Heights, Bowling Green, 42101-1080, Kentucky, USA.
Department of Plant Systems Biology, VIB, Ghent University, Gent, 9000, Belgium.
Sci Rep. 2017 Sep 12;7(1):11430. doi: 10.1038/s41598-017-11896-5.
The mechanism of gold nanoparticle formation and genes involved in such processes, especially Au transport in plants are not understood. Previous reports pointed to the probable role of COPT2 in Au transport based on the transcript accumulation of COPT2 under Au exposure. Here, we provide evidence revealing the additional role of COPT2 for Au mobilization in yeast and Arabidopsis. The COPT2 transcripts significantly accumulated in the root of Arabidopsis under Au exposure. The expression of COPT2 restores Cu uptake ability in ctr1Δctr3Δ mutants and leads to Au sensitivity in yeast, which is comparable to Cu in growth kinetics experiments. The metal measurement data showed that the Au level was increased in COPT2, expressing yeast cells compared to vector transformed control. The copt2 mutant of Arabidopsis displayed a similar growth pattern to that of Col-0 under Au treatment. However, a notable phenotypic difference was noticed in three-week-old plants treated with and without Au. Consistent with yeast, Au uptake was reduced in the copt2 mutant of Arabidopsis. Together, these results clearly reveal the Au uptake capability of COPT2 in yeast and Arabidopsis. This is the first report showing the potential role of any transporter towards uptake and accumulation of Au in plants.
金纳米颗粒形成的机制以及涉及这些过程的基因尚不清楚，特别是金在植物中的运输机制尚不清楚。先前的研究报告指出，基于 COPT2 在金暴露下的转录积累，COPT2 可能在金的运输中发挥作用。在这里，我们提供了证据，揭示了 COPT2 在酵母和拟南芥中对金动员的额外作用。在金暴露下，COPT2 的转录物在拟南芥的根部显著积累。COPT2 的表达恢复了 ctr1Δctr3Δ 突变体中的 Cu 摄取能力，并导致酵母对金的敏感性，这与生长动力学实验中的 Cu 相当。金属测量数据显示，与载体转化对照相比，在表达 COPT2 的酵母细胞中，金的水平增加。拟南芥的 copt2 突变体在金处理下表现出与 Col-0 相似的生长模式。然而，在处理和未处理的三周龄植物中，注意到了一个明显的表型差异。与酵母一致，拟南芥 copt2 突变体中的金摄取减少。总的来说，这些结果清楚地揭示了 COPT2 在酵母和拟南芥中对金的摄取能力。这是第一个表明任何转运蛋白在植物中摄取和积累金的潜在作用的报告。
