Ma Fei, Liu Wen-Jing, Zhang Qianyi, Zhang Chun-Yang
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China.
Chem Commun (Camb). 2017 Sep 21;53(76):10596-10599. doi: 10.1039/c7cc06290g.
Herein, we develop a simple fluorescence method for the sensitive detection of microRNAs based on duplex specific nuclease-assisted target recycling and pyrene excimer switching. This method is extremely sensitive with a detection limit of 0.58 fM, and it can be applied to absolutely detect endogenous microRNAs in cancer cells, holding great potential for clinical diagnosis and biomedical research studies.
在此，我们基于双链特异性核酸酶辅助的靶标循环和芘激基缔合物转换，开发了一种用于灵敏检测微小RNA的简单荧光方法。该方法极其灵敏，检测限为0.58 fM，可用于绝对检测癌细胞中的内源性微小RNA，在临床诊断和生物医学研究中具有巨大潜力。
