Zong Xiu-Hong, Deng Hong-Ping, Huang Qin, Yang Mou, Li Yun-Ting
School of Life Sciences, Southwest University, Key Laboratory of Eco-envrionments of Three Gores Reservoir Region of Ministry of Education, Chongqing Key Laboratory of Plant Ecology and Resources Research for Three Gorges Reservoir Region, Chongqing 400715, China.
Zhongguo Zhong Yao Za Zhi. 2016 Jun;41(12):2201-2207. doi: 10.4268/cjcmm20161205.
Traditional squash method was used to analyze chromosome number and karyotypes of four Scutellaria species in Chongqing Jinyun Mountain Natural Reserve: Scutellaria tsinyunensis, S.yunnanensis, S.franchetiana and S.indica.The result showed that the chromosome numbers were 26 except for S.franchetiana, which had 24 chromosomes.These species were all diploid with metacentric and submetacentric chromosomes.Their karyotypes were symmetrical and primitive.The karyotype formula of S.tsinyunensis is 2n=2x=26=24m+2sm, 1B type, As.k=55.28%; the karyotype formula of S.yunnanensis var.salicifolia is 2n=2x=26=26m, 1B type, As.k=56.11%; the karyotype formula of S.franchetiana is 2n=2x=24=20m+4sm, 2B type, As.k=58.50%; the karyotype formula of S.indica is 2n=2x=24=20m+4sm, 2B type, As.k=58.41%.The results were compared with the reported data of S.baicalensis and S.alaschanica.S.alaschanica is expected to be the most advanced one whereas S.tsinyunensis, and S.yunnanensis var. salicifolia primitive.These results are expected to provide some references to the origin and differentiation of genus Scutellaria.
采用传统压片法对重庆缙云山自然保护区的4种黄芩属植物进行染色体数目及核型分析,分别为缙云黄芩、滇黄芩、方枝黄芩和半枝莲。结果显示,除方枝黄芩染色体数目为24条外,其余3种染色体数目均为26条。这些物种均为二倍体,染色体类型为中着丝粒和近中着丝粒染色体,核型对称且原始。缙云黄芩的核型公式为2n=2x=26=24m+2sm,1B型,核型不对称系数As.k=55.28%;柳叶黄芩变种的核型公式为2n=2x=26=26m,1B型,As.k=56.11%;方枝黄芩的核型公式为2n=2x=24=20m+4sm,2B型,As.k=58.50%;半枝莲的核型公式为2n=2x=24=20m+4sm,2B型,As.k=58.41%。将这些结果与已报道的黄芩和阿拉善黄芩的数据进行比较,阿拉善黄芩可能是最进化的,而缙云黄芩和柳叶黄芩变种较为原始。这些结果有望为黄芩属的起源与分化提供参考。