Xu Wen-Feng, Pei Yue-Hu
Department of Pharmacy, Beijing Hospital, Beijing 100730, China.
Key Laboratory of Target-Based Drug Design and Research Under Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China.
Zhongguo Zhong Yao Za Zhi. 2016 Jun;41(12):2228-2234. doi: 10.4268/cjcmm20161210.
To study the chemical component groups with toxicity alleviation effect to Realgar in Niuhuang Jiedu tablet based on ¹H-NMR metabonomics. Twenty-four male Wistar rats were divided into four groups： control group, R group (treated with Realgar), RRSPG group (treated with Realgar, the root and rhizoma of Rheum palmatum, the root of Scutellaria baicalensis, the root of Platycodon grandiflorum and the root and rhizoma of Glycyrrhiza uralensis) and RC group (treated with total anthraquinones from the root and rhizoma of R. palmatum, total flavonoids from the root of S. baicalensis, total saponins from the root of P. grandiflorum, total flavonoids and saponins from the root and rhizoma of G. uralensis). Based on ¹H-NMR spectra of urine and serum from rats, PLS-DA was performed to identify different metabolic profiles.The metabolic profiles of R group were different from that of control group, while the metabolic profiles of RC group were almost similar to control group.Total anthraquinones from the root and rhizoma of R. palmatum, total flavonoids from the root of S. baicalensis, total saponins from the root of P. grandiflorum, total flavonoids and saponins from the root and rhizoma of G. uralensis regulated energy, choline and amino acid metabolism and gut flora disorder affected by realgar's toxicity.
基于¹H-NMR代谢组学研究牛黄解毒片中对雄黄具有减毒作用的化学成分群。将24只雄性Wistar大鼠分为四组：对照组、R组（给予雄黄）、RRSPG组（给予雄黄、大黄根茎、黄芩根、桔梗根和甘草根茎）和RC组（给予大黄根茎总蒽醌、黄芩根总黄酮、桔梗根总皂苷、甘草根茎总黄酮和总皂苷）。基于大鼠尿液和血清的¹H-NMR谱图，进行偏最小二乘判别分析（PLS-DA）以识别不同的代谢谱。R组的代谢谱与对照组不同，而RC组的代谢谱与对照组几乎相似。大黄根茎总蒽醌、黄芩根总黄酮、桔梗根总皂苷、甘草根茎总黄酮和总皂苷调节了受雄黄毒性影响的能量、胆碱和氨基酸代谢以及肠道菌群紊乱。
