Xu Wenfeng, Pei Yuehu, Xu Shuo, Wang Haifeng, Jin Pengfei
Department of Pharmacy, National Center of Gerontology, Beijing Hospital, Beijing 100730, China.
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
Evid Based Complement Alternat Med. 2018 Jan 23;2018:2154603. doi: 10.1155/2018/2154603. eCollection 2018.
Niuhuang Jiedu Tablet (NJT) is a classical formula in treating acute tonsillitis, pharyngitis, and so on. In the formula, significant level of as a potentially toxic element is contained. Our previous experiments revealed that it was less toxic for combined in NJT. However, the active fraction of this prescription with toxicity alleviation effect on was still obscure. NJT was divided into five different polar fractions (NJT-PET, NJT-25, NJT-50, NJT-75, and NJT-95), and we explored the toxicity alleviation effect on . Based on H NMR spectra of urine and serum from rats, PCA and PLS-DA were performed to identify different metabolic profiles. Liver and kidney histopathology examinations and serum clinical chemistry analysis were also performed. With pattern recognition analysis of metabolites in urine and serum, Realgar group showed a clear separation from control group, while the metabolic profiles of NJT-PET, NJT-25, NJT-50, and NJT-95 groups were similar to Realgar group, and the metabolic profiles of NJT and NJT-75 groups were very close to control group. Statistics results were confirmed by the histopathological examination and biochemical assay. The present work indicated that 75% EtOH fraction of NJT was the most valid fraction with the toxicity alleviation effect on .
牛黄解毒片（NJT）是治疗急性扁桃体炎、咽炎等疾病的经典方剂。该方剂中含有一定量的砷，砷是一种潜在的有毒元素。我们之前的实验表明，牛黄解毒片中的砷联合使用时毒性较小。然而，该方剂中具有减轻砷毒性作用的活性部位仍不清楚。将牛黄解毒片分为五个不同极性部位（NJT-PET、NJT-25、NJT-50、NJT-75和NJT-95），探讨其对砷的减毒作用。基于大鼠尿液和血清的核磁共振氢谱，采用主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）来识别不同的代谢谱。同时进行肝、肾组织病理学检查和血清临床化学分析。通过对尿液和血清中代谢物的模式识别分析，雄黄组与对照组有明显分离，而NJT-PET、NJT-25、NJT-50和NJT-95组的代谢谱与雄黄组相似，NJT组和NJT-75组的代谢谱与对照组非常接近。组织病理学检查和生化检测结果证实了统计结果。目前的研究表明，牛黄解毒片75%乙醇部位是对砷具有减毒作用的最有效部位。
