Kuang Hai-Xue, Wu Gao-Song, Liu Hua, Yu Yang, Wu Qiong, Wang Qiu-Hong, Yang Bing-You, Wang Zhi-Bin
Key Laboratory of Chinese Materia Medica(Ministry of Education), Heilongjiang University of Chinese Medicine, Heilongjiang Key Laboratory of Traditional Chinese Medicine Pharmacodynamic Material Bases, Harbin 150040, China.
Zhongguo Zhong Yao Za Zhi. 2016 Jun;41(12):2280-2283. doi: 10.4268/cjcmm20161218.
Twelve xanthones compounds were isolated from the ethanol extract of Gentianella acuta by means of reversed-phase preparative HPLC and various kinds of column chromatography including silica gel and ODS . Their structures were fully elucidated on the basis of MS, 1D and 2D-NMR data. The structures of xanthones were identified as 1, 7-dihydroxy-3-methoxyxanthone-7-O-β-D-glucopyranoside (1), swertiapuniside (2), 1, 3, 8- trihydroxy -4, 5-dimethoxyxanthone-1-O-β-D-glucopyranosyl(6→1)-O-β-D-glucopyranoside (3), 1, 2, 8-trihydroxy-5, 6-dimethoxyxanthone-2-O-β-D-glucopyranoside (4), 1, 3, 7, 8-tetrahydroxyxanthone-1-O-β-D-glucopyranoside (5), 1, 3, 5, 8-tetrahydroxy-5, 6, 7, 8-tetrahydroxanthone (6), 1, 3, 5-thihydroxyxanthone (7),1, 3, 5, 8-tetrahydroxyxanthone (8), 1, 2, 8-trihydroxy-5, 6-dimethoxyxanthone (9), bellidifolin (10), mangiferin (11), swertianolin (12). Compounds 1-9 were isolated from Gentianella genus for the first time.
通过反相制备高效液相色谱以及包括硅胶柱和十八烷基硅烷键合硅胶柱在内的各种柱色谱法，从尖叶假龙胆乙醇提取物中分离出12种呫吨酮化合物。基于质谱、一维和二维核磁共振数据对它们的结构进行了全面解析。这些呫吨酮化合物的结构被鉴定为1,7 - 二羟基 - 3 - 甲氧基呫吨酮 - 7 - O - β - D - 吡喃葡萄糖苷（1）、当药苷（2）、1,3,8 - 三羟基 - 4,5 - 二甲氧基呫吨酮 - 1 - O - β - D - 吡喃葡萄糖基（6→1）- O - β - D - 吡喃葡萄糖苷（3）、1,2,8 - 三羟基 - 5,6 - 二甲氧基呫吨酮 - 2 - O - β - D - 吡喃葡萄糖苷（4）、1,3,7,8 - 四羟基呫吨酮 - 1 - O - β - D - 吡喃葡萄糖苷（5）、1,3,5,8 - 四羟基 - 5,6,7,8 - 四氢呫吨酮（6）、1,3,5 - 三羟基呫吨酮（7）、1,3,5,8 - 四羟基呫吨酮（8）、1,2,8 - 三羟基 - 5,6 - 二甲氧基呫吨酮（9）、美丽日中花素（10）、芒果苷（11）、獐牙菜苦苷（12）。化合物1 - 9首次从假龙胆属植物中分离得到。
