Liu Qing, Wang Yong-Zhong, Jiang Hui, Han Yan-Quan, Li Yu-Xin
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230038, China.
The First Affiliated Hospital of Anhui University of Chinese Medicine, Grade 3 Laboratory of Traditional Chinese Medicine Preparation, State Administration of Traditional Chinese Medicine, Hefei 230031, China.
Zhongguo Zhong Yao Za Zhi. 2016 Jun;41(12):2321-2328. doi: 10.4268/cjcmm20161225.
To observe the effect of total saponins of Clematidis Radix et Rhizoma (TSCR) on serum metabolic profile changes in adjuvant arthritis(AA) rats, and explore its possible action mechanism for AA rats. The AA rat models were induced by Freund's complete adjuvant(FCA), and their histopathological changes were observed. Gas chromatography-time-of-flight mass spectrometry (GC-TOF-MS), principal component analysis(PCA) and partial least squares-discriminant analysis (PLS-DA) were employed to analyze the metabolic profile among normal group, AA model group and TSCR group. Potential biomarkers in the serum were screened based on the variable importance projection(VIP) value>1, P<0.05. As compared with the normal group, 17 potential biomarkers such as aspartic acid, inositol and phenylacetaldehyde were found and identified in the serum of model group rats. As compared with the model group, the above biomarkers were regulated nearly to a normal state after TSCR administration for 16 days. Metabolomic analysis revealed that the total saponins of Clematidis Radix et Rhizoma has a certain therapeutic effect for AA rats, and the mechanism may be related to regulation of lipid metabolism, amino acid metabolism and energy metabolism.
观察威灵仙总皂苷（TSCR）对佐剂性关节炎（AA）大鼠血清代谢谱变化的影响，并探讨其对AA大鼠可能的作用机制。采用弗氏完全佐剂（FCA）诱导建立AA大鼠模型，观察其组织病理学变化。运用气相色谱-飞行时间质谱（GC-TOF-MS）、主成分分析（PCA）和偏最小二乘判别分析（PLS-DA）对正常组、AA模型组和TSCR组的代谢谱进行分析。基于变量重要性投影（VIP）值>1、P<0.05筛选血清中的潜在生物标志物。与正常组相比，模型组大鼠血清中发现并鉴定出17种潜在生物标志物，如天冬氨酸、肌醇和苯乙醛等。与模型组相比，TSCR给药16天后上述生物标志物几乎被调节至正常状态。代谢组学分析表明，威灵仙总皂苷对AA大鼠具有一定的治疗作用，其机制可能与调节脂质代谢、氨基酸代谢和能量代谢有关。
