Samoylova Tatiana I, Braden Timothy D, Spencer Jennifer A, Bartol Frank F
Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
Curr Med Chem. 2017 Nov 20;24(35):3907-3920. doi: 10.2174/0929867324666170911160426.
Population control of domestic, wild, invasive, and captive animal species is a global issue of importance to public health, animal welfare and the economy. There is pressing need for effective, safe, and inexpensive contraceptive technologies to address this problem. Contraceptive vaccines, designed to stimulate the immune system in order to block critical reproductive events and suppress fertility, may provide a solution. Filamentous bacteriophages can be used as platforms for development of such vaccines.
In this review authors highlight structural and immunogenic properties of filamentous phages, and discuss applications of phage-peptide vaccines for advancement of immunocontraception technology in animals.
Phages can be engineered to display fusion (non-phage) peptides as coat proteins. Such modifications can be accomplished via genetic manipulation of phage DNA, or by chemical conjugation of synthetic peptides to phage surface proteins. Phage fusions with antigenic determinants induce humoral as well as cell-mediated immune responses in animals, making them attractive as vaccines. Additional advantages of the phage platform include environmental stability, low cost, and safety for immunized animals and those administering the vaccines.
Filamentous phages are viable platforms for vaccine development that can be engineered with molecular and organismal specificity. Phage-based vaccines can be produced in abundance at low cost, are environmentally stable, and are immunogenic when administered via multiple routes. These features are essential for a contraceptive vaccine to be operationally practical in animal applications. Adaptability of the phage platform also makes it attractive for design of human immunocontraceptive agents.
对家养、野生、入侵及圈养动物物种进行种群控制是一个对公共卫生、动物福利和经济都至关重要的全球性问题。迫切需要有效、安全且廉价的避孕技术来解决这一问题。旨在刺激免疫系统以阻断关键生殖事件并抑制生育能力的避孕疫苗或许能提供一种解决方案。丝状噬菌体可被用作此类疫苗的开发平台。
在本综述中，作者强调了丝状噬菌体的结构和免疫原性特性，并讨论了噬菌体 - 肽疫苗在推进动物免疫避孕技术方面的应用。
噬菌体可通过基因工程改造，使其将融合（非噬菌体）肽展示为外壳蛋白。这种修饰可通过对噬菌体DNA进行基因操作来实现，或者通过将合成肽与噬菌体表面蛋白进行化学偶联来完成。与抗原决定簇融合的噬菌体可在动物体内诱导体液免疫以及细胞介导的免疫反应，使其成为有吸引力的疫苗。噬菌体平台的其他优势包括环境稳定性、低成本以及对免疫动物和疫苗接种者的安全性。
丝状噬菌体是可行的疫苗开发平台，可通过分子和生物特异性进行工程改造。基于噬菌体的疫苗可以低成本大量生产，具有环境稳定性，并且通过多种途径给药时具有免疫原性。这些特性对于避孕疫苗在动物应用中实际可行至关重要。噬菌体平台的适应性也使其在人类免疫避孕剂的设计方面具有吸引力。
