Chen Jianliang, Li Shu, Zhou Shu, Cao Shouji, Lou Yun, Shen Haiyuan, Yin Jie, Li Guoqiang
Department of General Surgery, People' Hospital, Jingjiang 214500; Department of Liver Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210009; Department of Liver Surgery, Key Laboratory of Living Donor Liver Transplantation of Ministry of Public Health, Nanjing 210009, China.
Department of Liver Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210009; Department of Liver Surgery, Key Laboratory of Living Donor Liver Transplantation of Ministry of Public Health, Nanjing 210009, China.
J Cancer Res Ther. 2017;13(4):651-659. doi: 10.4103/jcrt.JCRT_491_17.
In this study, we characterized the expression of 32 other kinesin superfamily proteins (KIFs) and analyzed their association with the progression and prognosis of hepatocellular carcinoma (HCC).
The data from 295 HCC patients from The Cancer Genome Atlas were included in the study. An independent t-test was used to compare the KIF levels in HCC and adjacent tissues. Pearson's Chi-square test was used to assess the relationships of KIF expression with tumor biomarkers and clinicopathological parameters. Kaplan-Meier plots and log-rank tests were used to analyze survival, and univariate and multivariate analyses were used to identify independent prognostic factors.
The expressions of 32 KIFs were compared between HCC and adjacent nontumor tissues. Among them, 12 KIFs showed no statistical significance, 17 KIFs were upregulated, and three KIFs were downregulated in tumor tissues. The levels of some KIFs were markedly correlated with that of biomarkers for the S phase and proliferation. KIF2A and KIFC3 expression was positively associated with biomarkers for cell invasion and migration. Some KIF overexpression was significantly associated with neoplastic pathological grade and tumor-node-metastasis staging. Furthermore, KIF2C, KIF4A, and KIF11 overexpression were significantly associated with shorter relapse-free survival times. KIF2A, KIF2C, KIF3A, KIF4B, KIF11, KIF15, KIFC1, and KIFC3 overexpression was associated with shorter overall survival (OS) times, whereas higher expression of KIF19 was associated with a longer OS time. Further multivariate analyses suggested that only KIF4B was an independent prognostic factor for HCC.
Most overexpressions of abnormal KIFs were significantly associated with HCC progression and prognosis, indicating that KIFs could be prognostic and therapeutic biomarkers for HCC. However, it is necessary to further study the function of KIFs and their mechanisms involved in HCC.
在本研究中，我们对另外32种驱动蛋白超家族蛋白（KIFs）的表达进行了表征，并分析了它们与肝细胞癌（HCC）进展和预后的关系。
本研究纳入了来自癌症基因组图谱的295例HCC患者的数据。采用独立样本t检验比较HCC组织和癌旁组织中的KIF水平。使用Pearson卡方检验评估KIF表达与肿瘤生物标志物及临床病理参数之间的关系。采用Kaplan-Meier曲线和对数秩检验分析生存率，并进行单因素和多因素分析以确定独立的预后因素。
比较了HCC组织和癌旁非肿瘤组织中32种KIFs的表达。其中，12种KIFs无统计学意义，17种KIFs在肿瘤组织中上调，3种KIFs在肿瘤组织中下调。一些KIFs的水平与S期和增殖的生物标志物显著相关。KIF2A和KIFC3的表达与细胞侵袭和迁移的生物标志物呈正相关。一些KIF的过表达与肿瘤病理分级和肿瘤-淋巴结-转移分期显著相关。此外，KIF2C、KIF4A和KIF11的过表达与无复发生存期显著缩短相关。KIF2A、KIF2C、KIF3A、KIF4B、KIF11、KIF15、KIFC1和KIFC3的过表达与总生存期（OS）缩短相关，而KIF19的高表达与较长的OS时间相关。进一步的多因素分析表明，只有KIF4B是HCC的独立预后因素。
大多数异常KIFs的过表达与HCC的进展和预后显著相关，表明KIFs可能是HCC的预后和治疗生物标志物。然而，有必要进一步研究KIFs的功能及其在HCC中的作用机制。
