Department of Basic Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China.
Department of Animal Medicine, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, P.R. China.
Mol Med Rep. 2017 Nov;16(5):6518-6525. doi: 10.3892/mmr.2017.7421. Epub 2017 Sep 5.
The present study aimed to compare the expression levels of excitatory amino acid transporters (EAATs) and growth status of piglets weaned at 10‑20 days after birth with suckling piglets. A total of 40 hybrid piglets (Landrace x Large White x Duroc) born to 40 different sows, with similar body weight were selected for the present study. They were randomly divided into two groups (n=20 per group): Control group (suckling piglets) and experimental group (weaned piglets, reared in isolation). The experiment lasted for 10 days. At the end of the experiment, 12 piglets were randomly selected from each group and the jejunum and the ileum were collected in order to determine excitatory amino acid carrier 1 (EAAC1) expression levels and free amino acid content. The present study determined that early weaning significantly reduced EAAC1 gene and protein (57 and 73 kDa) expression levels and glutamate transporter associate protein 3‑18 (GTRAP3‑18; 50 kDa) in the jejunum and the ileum compared with the suckling group (P<0.05). Weaning led to an increased content of free glutamic acid (Glu) and total amino acids in the jejunum; however, content of free Glu and total amino acids in the ileum was significantly reduced (P<0.05). Early weaning reduced the expression of EAAC1 and GTRAP3‑18, which was possibly due to the amino acid absorption and transport disorder in the small intestine due to the Glu deficiency.
本研究旨在比较在出生后 10-20 天断奶的仔猪与哺乳仔猪的兴奋性氨基酸转运体（EAATs）表达水平和生长状况。选择 40 头来自 40 头不同母猪的杂交仔猪（长白猪×大白猪×杜洛克），体重相似，进行本研究。它们被随机分为两组（每组 20 头）：对照组（哺乳仔猪）和实验组（断奶仔猪，单独饲养）。实验持续 10 天。实验结束时，从每组中随机选择 12 头仔猪，收集空肠和回肠，以确定兴奋性氨基酸载体 1（EAAC1）表达水平和游离氨基酸含量。本研究确定，早期断奶显著降低了空肠和回肠中 EAAC1 基因和蛋白（57 和 73 kDa）表达水平，以及谷氨酸转运体相关蛋白 3-18（GTRAP3-18；50 kDa）（P<0.05）。断奶导致空肠中游离谷氨酸（Glu）和总氨基酸含量增加；然而，回肠中游离 Glu 和总氨基酸含量显著降低（P<0.05）。早期断奶降低了 EAAC1 和 GTRAP3-18 的表达，这可能是由于 Glu 缺乏导致小肠中氨基酸吸收和转运紊乱。
