Department of Obstetrics, The Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, P.R. China.
Mol Med Rep. 2017 Nov;16(5):6650-6673. doi: 10.3892/mmr.2017.7462. Epub 2017 Sep 11.
The microRNA (miRNA) profiles of placentas complicated with selective intrauterine growth restriction (sIUGR) are unknown. In the present study, the sIUGR‑associated placental miRNA expression was investigated using microarray and confirmatory reverse transcriptase‑quantitative polymerase chain reaction studies. Placenta samples around the individual insertion region for each umbilical cord were collected from monochorionic twins complicated with (n=17) or without sIUGR (control, n=16). miRNA profile analysis was performed on two sIUGR cases and one control using an Affymetrix microRNA 4.0 Array system. A total of 14 miRNAs were identified to be specifically differentially expressed (7 upregulated and 7 downregulated) among larger twins of sIUGR cases compared with smaller twins of sIUGR cases. The target genes of the identified miRNAs participate in organ size, cell differentiation, cell proliferation and migration. In addition, according to the miRNA‑pathway network analysis, key miRNAs and pathways (transforming growth factor‑β, mitogen‑activated protein kinase and Wnt) were identified to be associated with the pathogenesis of sIUGR. To the best of our knowledge, the results of the current study have provided the most complete miRNA profiles and the most detailed miRNA regulatory networks of placental tissues complicated with sIUGR.
患有选择性宫内生长受限（sIUGR）的胎盘的 microRNA（miRNA）谱尚不清楚。在本研究中，通过微阵列和确认性逆转录-定量聚合酶链反应研究研究了与 sIUGR 相关的胎盘 miRNA 表达。从伴有（n=17）或不伴有 sIUGR（对照，n=16）的单绒毛膜双胞胎的脐带每个插入区域周围采集胎盘样本。使用 Affymetrix microRNA 4.0 Array 系统对两个 sIUGR 病例和一个对照进行 miRNA 谱分析。与 sIUGR 病例的较小双胞胎相比，在 sIUGR 病例的较大双胞胎中，鉴定出 14 种 miRNA 特异性差异表达（7 种上调和 7 种下调）。鉴定出的 miRNA 的靶基因参与器官大小、细胞分化、细胞增殖和迁移。此外，根据 miRNA-通路网络分析，确定关键 miRNA 和通路（转化生长因子-β、丝裂原活化蛋白激酶和 Wnt）与 sIUGR 的发病机制相关。据我们所知，本研究的结果提供了最完整的 miRNA 图谱和最详细的与 sIUGR 相关的胎盘组织 miRNA 调控网络。
