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2'-O-甲基-5-羟甲基胞苷、5-羟甲基胞苷和5-甲酰基胞苷单体的合成及其在RNA寡核苷酸中的多重掺入

Synthesis and Multiple Incorporations of 2'-O-Methyl-5-hydroxymethylcytidine, 5-Hydroxymethylcytidine and 5-Formylcytidine Monomers into RNA Oligonucleotides.

作者信息

Tanpure Arun A, Balasubramanian Shankar

机构信息

Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.

Cancer Research (UK), Cambridge Institute, Li Ka Shing Centre University of Cambridge, Robinson Way, Cambridge, CB2 0RE, UK.

出版信息

Chembiochem. 2017 Nov 16;18(22):2236-2241. doi: 10.1002/cbic.201700492. Epub 2017 Oct 9.

DOI:10.1002/cbic.201700492
PMID:28901692
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5779611/
Abstract

The synthesis of 2'-O-methyl-5-hydroxymethylcytidine (hm Cm), 5-hydroxymethylcytidine (hm C) and 5-formylcytidine (f C) phosphoramidite monomers has been developed. Optimisation of mild post-synthetic deprotection conditions enabled the synthesis of RNA containing all four naturally occurring cytosine modifications (hm Cm, hm C, f C plus 5-methylcytosine). Given the considerable interest in RNA modifications and epitranscriptomics, the availability of synthetic monomers and RNAs containing these modifications will be valuable for elucidating their biological function(s).

摘要

已开发出2'-O-甲基-5-羟甲基胞苷（hm Cm）、5-羟甲基胞苷（hm C）和5-甲酰基胞苷（f C）亚磷酰胺单体的合成方法。温和的合成后脱保护条件的优化使得能够合成包含所有四种天然存在的胞嘧啶修饰（hm Cm、hm C、f C以及5-甲基胞嘧啶）的RNA。鉴于对RNA修饰和表观转录组学的广泛关注，含有这些修饰的合成单体和RNA的可得性对于阐明它们的生物学功能将具有重要价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a48/5820757/6e72374b146f/CBIC-18-2236-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a48/5820757/ba76c4823d5d/CBIC-18-2236-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a48/5820757/709933a53621/CBIC-18-2236-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a48/5820757/7b668bda5259/CBIC-18-2236-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a48/5820757/7df4402a1c80/CBIC-18-2236-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a48/5820757/4ecafe8f3afa/CBIC-18-2236-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a48/5820757/6e72374b146f/CBIC-18-2236-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a48/5820757/ba76c4823d5d/CBIC-18-2236-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a48/5820757/709933a53621/CBIC-18-2236-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a48/5820757/7b668bda5259/CBIC-18-2236-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a48/5820757/7df4402a1c80/CBIC-18-2236-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a48/5820757/4ecafe8f3afa/CBIC-18-2236-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a48/5820757/6e72374b146f/CBIC-18-2236-g002.jpg

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