Lü Zhen-Zhen, Li Yang, Zhou Yao, Cui Heng-Lin, Li Zheng-Rong
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Department of Cell Biology, Nanjing Medical University, Nanjing 210029, PR China.
Int J Syst Evol Microbiol. 2017 Oct;67(10):4095-4099. doi: 10.1099/ijsem.0.002259. Epub 2017 Sep 13.
Halophilic archaeal strain ZS-54-S2 was isolated from Zhoushan marine solar saltern, China. Cells were rod-shaped, Gram-stain-negative and formed red-pigmented colonies on an agar plate. Strain ZS-54-S2 was able to grow at 20-50 °C (optimum 35 °C), at 1.7-4.8 M NaCl (optimum 3.9 M), at 0.005-1.0 M MgCl2 (optimum 0.05 M) and at pH 5.0-9.5 (optimum pH 7.0). The cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was found to be 5 % (w/v). The major polar lipids of the strain were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, two glycolipids, which were chromatographically identical to sulfated galactosyl mannosyl galactofuranosyl glucosyl diether and galactosyl mannosyl glucosyl diether, and an unidentified glycolipid, which was chromatographically identical to one detected in Halobacterium salinarum ATCC 33171. The 16S rRNA gene and rpoB' gene of strain ZS-54-S2 were phylogenetically related to the corresponding genes of Halobacterium noricense JCM 15102 (97.5 % and 90.6 % relatedness, respectively), Halobacterium jilantaiense CGMCC 1.5337 (96.9 and 91.2 %), Halobacterium rubrum CGMCC 1.12575 (96.8 and 90.3 %) and Halobacterium salinarum CGMCC 1.1958 (96.5 and 88.4 %). The DNA G+C content of strain ZS-54-S2 was 66.7 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain ZS-54-S2 (=CGMCC 1.12562=JCM 30038) represents a new species of Halobacterium, for which the name Halobacteriumlitoreum sp. nov. is proposed.
嗜盐古菌菌株ZS - 54 - S2从中国舟山海洋太阳能盐场分离得到。细胞呈杆状，革兰氏染色阴性，在琼脂平板上形成红色菌落。菌株ZS - 54 - S2能够在20 - 50°C（最适温度35°C）、1.7 - 4.8 M NaCl（最适浓度3.9 M）、0.005 - 1.0 M MgCl2（最适浓度0.05 M）以及pH 5.0 - 9.5（最适pH 7.0）的条件下生长。细胞在蒸馏水中会裂解，防止细胞裂解的最低NaCl浓度为5%（w/v）。该菌株的主要极性脂类为磷脂酸、磷脂酰甘油、磷脂酰甘油磷酸甲酯、两种糖脂（其在色谱上与硫酸化半乳糖基甘露糖基半乳糖呋喃糖基葡萄糖基二醚和半乳糖基甘露糖基葡萄糖基二醚相同）以及一种未鉴定的糖脂（其在色谱上与盐生盐杆菌ATCC 33171中检测到的一种糖脂相同）。菌株ZS - 54 - S2的16S rRNA基因和rpoB'基因在系统发育上与诺氏盐杆菌JCM 15102的相应基因相关（相似度分别为97.5%和90.6%）、吉兰泰盐杆菌CGMCC 1.5337（96.9%和91.2%）、红色盐杆菌CGMCC 1.12575（96.8%和90.3%）以及盐生盐杆菌CGMCC 1.1958（96.5%和88.4%）。菌株ZS - 54 - S2的DNA G + C含量为66.7 mol%。其表型、化学分类和系统发育特性表明，菌株ZS - 54 - S2（=CGMCC 1.12562 = JCM 30038）代表盐杆菌属的一个新物种，为此提出新名称嗜盐盐场盐杆菌（Halobacteriumlitoreum sp. nov.）。
