Zhou Yao, Li Yang, Lü Zhen-Zhen, Cui Heng-Lin
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
Arch Microbiol. 2017 Dec;199(10):1431-1435. doi: 10.1007/s00203-017-1420-z. Epub 2017 Aug 4.
Halophilic archaeal strain ZS-47-S was isolated from Zhoushan marine solar saltern, China. Cells were pleomorphic, stained Gram-negative, and formed red-pigmented colonies on agar plate. Strain ZS-47-S was able to grow at 20-50 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 3.4 M), at 0.005-1.0 M MgCl (optimum 0.03 M), and at pH 5.5-9.5 (optimum pH 6.5-7.5). The cells lysed in distilled water and the minimal NaCl concentration to prevent cell-lysis was 5% (w/v). The major polar lipids were CC and CC diether derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, glucosyl mannosyl glucosyl diether, and three unidentified glycolipids. The 16S rRNA gene and rpoB' gene of strain ZS-47-S were phylogenetically related to the corresponding genes of Halomarina oriensis JCM 16495 (98.57 and 92.94% similarities, respectively) and Halomarina salina CGMCC 1.12543 (97.96 and 93.65% similarities, respectively). The DNA G + C content of strain ZS-47-S was 64.6 mol % (T ). The phenotypic, chemotaxonomic, and phylogenetic properties suggested that strain ZS-47-S (=CGMCC 1.12563 = JCM 30037) represents a new species of Halomarina, for which the name Halomarina rubra sp. nov. is proposed.
嗜盐古菌菌株ZS-47-S是从中国舟山海洋太阳能盐场分离得到的。细胞呈多形性，革兰氏染色阴性，在琼脂平板上形成红色菌落。菌株ZS-47-S能够在20-50°C（最适温度37°C）、0.9-4.8M NaCl（最适浓度3.4M）、0.005-1.0M MgCl（最适浓度0.03M）以及pH 5.5-9.5（最适pH 6.5-7.5）的条件下生长。细胞在蒸馏水中会裂解，防止细胞裂解的最低NaCl浓度为5%（w/v）。主要极性脂类为磷脂酰甘油、磷脂酰甘油磷酸甲酯、磷脂酰甘油硫酸酯、葡萄糖基甘露糖基葡萄糖基二醚的CC和CC二醚衍生物，以及三种未鉴定的糖脂。菌株ZS-47-S的16S rRNA基因和rpoB'基因在系统发育上分别与 Oriensis盐海嗜盐菌JCM 16495的相应基因（相似性分别为98.57%和92.94%）以及盐盐湖嗜盐菌CGMCC 1.12543的相应基因（相似性分别为97.96%和93.65%）相关。菌株ZS-47-S的DNA G + C含量为64.6 mol%（T）。表型、化学分类和系统发育特性表明，菌株ZS-47-S（=CGMCC 1.12563 = JCM 30037）代表盐海嗜盐菌属的一个新物种，为此提出了新名称红色盐海嗜盐菌（Halomarina rubra sp. nov.）。
