Lima Elaine Cristina Bomfim de, Barbosa Walter Lins, Brito Maria Edileuza Felinto de, Melo Fábio Lopes de, Brandão Sinval Pinto, Medeiros Zulma Maria de
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Recife, Pernambuco, Brazil.
Universidade de Pernambuco, Instituto de Ciências Biológicas, Recife, Pernambuco, Brazil.
Rev Inst Med Trop Sao Paulo. 2017 Aug 24;59:e48. doi: 10.1590/S1678-9946201759048.
Visceral leishmaniasis, associated with HIV/AIDS coinfection, is becoming a more aggressive disease, complicating an accurate prognosis. A 21-year-old HIV-positive female presenting with clinical features of visceral leishmaniasis was enrolled in this study. Bone marrow cytology, Novy-MacNeal-Nicolle culture and kDNA PCR of peripheral blood were all positive. Typing methods, multilocus enzyme electrophoresis and ITS1-RFLP PCR of peripheral blood confirmed infection by Leishmania (L.) infantum chagasi . PCR has proved to be safer and more affordable than other characterization methods; ITS1-RFLP PCR can diagnose and type Leishmania spp. in both endemic and non-endemic areas, favoring the prognosis and allowing the appropriate treatment of patients.
与艾滋病毒/艾滋病合并感染相关的内脏利什曼病正成为一种更具侵袭性的疾病，使准确的预后变得复杂。一名出现内脏利什曼病临床特征的21岁艾滋病毒阳性女性被纳入本研究。骨髓细胞学检查、诺维-麦克尼尔-尼科尔培养以及外周血的kDNA聚合酶链反应均呈阳性。外周血的分型方法、多位点酶电泳和ITS1-RFLP聚合酶链反应证实感染了婴儿利什曼原虫（Leishmania (L.) infantum chagasi）。聚合酶链反应已被证明比其他鉴定方法更安全、更经济；ITS1-RFLP聚合酶链反应可在流行区和非流行区诊断利什曼原虫属并进行分型，有利于预后并能对患者进行适当治疗。
