da Silva Thais Almeida Marques, Gomes Luciana Inácia, Oliveira Edward, Coura-Vital Wendel, Silva Letícia de Azevedo, Pais Fabiano Sviatopolk-Mirsky, Ker Henrique Gama, Reis Alexandre Barbosa, Rabello Ana, Carneiro Mariangela
Laboratório de Pesquisas Clínicas, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brasil.
Laboratório de Epidemiologia das Doenças Infecciosas e Parasitárias, Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brasil.
Parasit Vectors. 2015 Apr 15;8:226. doi: 10.1186/s13071-015-0837-y.
Certain municipalities in the Belo Horizonte Metropolitan Area (BHMA), Minas Gerais, Brazil, have the highest human visceral leishmaniasis (VL) mortality rates in the country and also demonstrate high canine seropositivity. In Brazil, the etiologic agent of VL is Leishmania (Leishmania) infantum. The aim of this study was to evaluate the intraspecific genetic variability of parasites from humans and from dogs with different clinical forms of VL in five municipalities of BHMA using PCR-RFLP and two target genes: kinetoplast DNA (kDNA) and gp63.
In total, 45 samples of DNA extracted from clinical samples (n = 35) or L. infantum culture (n = 10) were evaluated. These samples originated from three groups: adults (with or without Leishmania/HIV co-infection; n = 14), children (n = 18) and dogs (n = 13). The samples were amplified for the kDNA target using the MC1 and MC2 primers (447 bp), while the Sg1 and Sg2 (1330 bp) primers were used for the gp63 glycoprotein target gene.
The restriction enzyme patterns of all the samples tested were monomorphic.
These findings reveal a high degree of genetic homogeneity for the evaluated gene targets among L. infantum samples isolated from different hosts and representing different clinical forms of VL in the municipalities of BHMA studied.
巴西米纳斯吉拉斯州贝洛奥里藏特大都市区（BHMA）的某些城市，人类内脏利什曼病（VL）死亡率在该国最高，犬类血清阳性率也很高。在巴西，VL的病原体是婴儿利什曼原虫（Leishmania (Leishmania) infantum）。本研究的目的是使用PCR-RFLP和两个靶基因：动质体DNA（kDNA）和gp63，评估BHMA五个城市中患有不同临床形式VL的人类和犬类寄生虫的种内遗传变异性。
总共评估了45份从临床样本（n = 35）或婴儿利什曼原虫培养物（n = 10）中提取的DNA样本。这些样本来自三组：成人（有或无利什曼原虫/HIV合并感染；n = 14）、儿童（n = 18）和犬类（n = 13）。使用MC1和MC2引物（447 bp）对kDNA靶标进行扩增，而使用Sg1和Sg2（1330 bp）引物对gp63糖蛋白靶标基因进行扩增。
所有测试样本的限制性酶切图谱均为单态性。
这些发现揭示了在BHMA研究城市中，从不同宿主分离的、代表不同临床形式VL的婴儿利什曼原虫样本中，所评估的基因靶标具有高度的遗传同质性。
