State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing, 100029, China.
Langmuir. 2018 Jan 23;34(3):1100-1108. doi: 10.1021/acs.langmuir.7b02608. Epub 2017 Sep 26.
There is an urgent demand to construct multiplexed biomolecular patterns to obtain more biological information from a single experiment. However, with only limited reports focusing on defective top-down approaches, challenges remain to develop a bottom-up strategy for multiplexed patterning. To this end, a novel strategy has been proposed to fabricate multiplexed DNA patterns via macroscopic assembly through combined precise magnetic manipulation and DNA hybridization-driven self-assembly. Therefore, a multiplexed DNA pattern composed of glass fibers loaded with multiple specific strands of DNA was constructed, and its potential application in simultaneous detection of multiplex target DNA was demonstrated. Moreover, the fabricated multiplexed DNA pattern shows an erasable behavior because the hybridized DNA can be disassembled by strand displacement.
目前迫切需要构建多重生物分子模式，以便从单个实验中获取更多的生物学信息。然而，由于仅有有限的报告集中在有缺陷的自上而下方法上，因此仍然存在开发用于多重图案化的自下而上策略的挑战。为此，提出了一种通过宏观组装来制造多重 DNA 图案的新策略，该策略通过精确的磁操作和 DNA 杂交驱动的自组装相结合来实现。因此，构建了由加载有多条特定 DNA 链的玻璃纤维组成的多重 DNA 图案，并证明了其在同时检测多重靶 DNA 中的潜在应用。此外，由于杂交的 DNA 可以通过链置换来解链，因此所构建的多重 DNA 图案具有可擦除的特性。
