Mullock B M, Hinton R H, Peppard J V, Slot J W, Luzio J P
Deparment of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, U.K.
Cell Biochem Funct. 1987 Oct;5(4):235-43. doi: 10.1002/cbf.290050402.
The endosome is an intracellular, acidic, membrane-bound, subcellular compartment to which endocytosed ligands, receptors and plasma membrane proteins are conveyed before sorting and delivery to destinations elsewhere in the cell. The preparative isolation of elements of this compartment has been achieved successfully using various appropriate combinations of density gradient ultracentrifugation, electrophoretic, gel filtration and immunoaffinity techniques. These methods for isolating endosome fractions are reviewed together with the difficulties of establishing markers for such fractions. The isolation of an endosome fraction from the pathway of polymeric IgA transcytosis in rat liver is discussed to exemplify successful isolation procedures and appropriate subcellular markers.
内涵体是一种细胞内的、酸性的、膜结合的亚细胞区室，内吞的配体、受体和质膜蛋白在分选并输送到细胞内其他目的地之前会被转运到该区域。利用密度梯度超速离心、电泳、凝胶过滤和免疫亲和技术的各种适当组合，已成功实现了对该区室成分的制备性分离。本文综述了这些分离内涵体组分的方法，以及为此类组分建立标志物的困难。讨论了从大鼠肝脏中多聚免疫球蛋白A转胞吞途径分离内涵体组分的过程，以举例说明成功的分离程序和合适的亚细胞标志物。
