Class 1 integrons and plasmid-mediated multiple resistance genes of the species from pediatric patient of a university hospital in Taiwan.

BACKGROUND

The species usually causes infection between humans and livestock interaction via livestock breeding. The studies of the species thus far in all clinical isolates were to show the many kinds of antibiotic phenomenon that were produced. Their integrons cause the induction of antibiotic resistance between bacterial species in the species.

RESULTS

The bacterial strains from the diarrhea of pediatric patient which isolated by China Medical University Hospital storage bank. These isolates were identified by MALDI-TOF mass spectrometry. The anti-microbial susceptibility test showed that Campylobacter species resistant to cefepime, streptomycin, tobramycin and trimethoprim/sulfamethoxazole (all and isolates), ampicillin (89% of ; 75% of ), cefotaxime (78% of ; 100% of ), nalidixic acid (78% of ; 100% of ), tetracycline (89% of ; 25% ), ciprofloxacin (67% of ; 50% ), kanamycin (33% of ; 75% ) and the isolate resisted to ampicillin, cefotaxime, nalidixic acid, tetracycline, ciprofloxacin, kanamycin by disc-diffusion method. The effect for ciprofloxacin and tetracycline of the species was tested using an E-test. The , and genes were detected by PCR assay. According to the sequencing analysis (type I: -- genes and type II: gene), the cassette type was identified. The most common gene cassette type (type I: 9 and 2 isolates; type II: 1 isolates) was found in 12 class I integrase-positive isolates.

CONCLUSIONS

Our results suggested an important information in the latency of species with resistance genes, and irrational antimicrobial use should be concerned.