Baetscher Diana S, Hasselman Daniel J, Reid Kerry, Palkovacs Eric P, Garza John Carlos
Department of Ocean Sciences University of California Santa Cruz CA USA.
Southwest Fisheries Science Center National Marine Fisheries Service Santa Cruz CA USA.
Ecol Evol. 2017 Jul 18;7(17):6638-6648. doi: 10.1002/ece3.3215. eCollection 2017 Sep.
Freshwater habitat alteration and marine fisheries can affect anadromous fish species, and populations fluctuating in size elicit conservation concern and coordinated management. We describe the development and characterization of two sets of 96 single nucleotide polymorphism (SNP) assays for two species of anadromous alosine fishes, alewife and blueback herring (collectively known as river herring), that are native to the Atlantic coast of North America. We used data from high-throughput DNA sequencing to discover SNPs and then developed molecular genetic assays for genotyping sets of 96 individual loci in each species. The two sets of assays were validated with multiple populations that encompass both the geographic range and the known regional genetic stocks of both species. The SNP panels developed herein accurately resolved the genetic stock structure for alewife and blueback herring that was previously identified using microsatellites and assigned individuals to regional stock of origin with high accuracy. These genetic markers, which generate data that are easily shared and combined, will greatly facilitate ongoing conservation and management of river herring including genetic assignment of marine caught individuals to stock of origin.
淡水生境改变和海洋渔业会影响溯河产卵鱼类，种群数量波动引发了保护关注和协同管理。我们描述了针对两种溯河产卵的西鲱属鱼类——美洲西鲱和蓝背西鲱（统称为河鲱）开发并表征两组96个单核苷酸多态性（SNP）检测方法的过程，这两种鱼原产于北美大西洋沿岸。我们利用高通量DNA测序数据发现SNP，然后开发分子遗传检测方法，用于对每个物种的96个个体位点进行基因分型。这两组检测方法在多个种群中得到验证，这些种群涵盖了两个物种的地理范围和已知的区域遗传种群。本文开发的SNP面板准确解析了之前使用微卫星鉴定出的美洲西鲱和蓝背西鲱的遗传种群结构，并以高精度将个体分配到其区域起源种群。这些遗传标记产生的数据易于共享和整合，将极大地促进河鲱当前的保护和管理工作，包括将海洋捕获个体进行遗传溯源。
