火鸡肌胃肌间神经丛的化学去神经支配

Chemical denervation of the myenteric plexus of the muscular stomach of turkeys.

Chaplin S B, Duke G E, Hunt H, Degernes L A

Department of Veterinary Biology, University of Minnesota, St Paul 55108.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1987;88(1):201-7. doi: 10.1016/0742-8413(87)90069-7.

Abstract

The thin caudoventral muscle (TCM) of the muscular stomach of domestic turkeys was surgically exposed and painted with solutions of saline or 0.1, 0.25, 0.5, and 1.0% benzalkonium chloride (BC), a cationic surfactant shown to irreversibly damage neurons but not muscle tissue in mammals. 2. Following fluoroscopic observations of gastric motility for 2 weeks, turkeys were euthanized, the entire muscular stomach was excised and weighed, and serial frozen sections of the TCM were taken for evaluation of the number and size of neurons in the myenteric plexus. 3. Treatment with 0.5 and 1.0% BC resulted in loss of motility in the TCM, significant hypertrophy (P less than 0.001) of the CTM, a 70% decrease in number and 60% decrease in size of myenteric neurons, and a 4-fold increase in thickness of the serosa, compared with saline-treated controls.

摘要

对家火鸡肌胃的薄尾腹侧肌（TCM）进行手术暴露，并用生理盐水或0.1%、0.25%、0.5%和1.0%苯扎氯铵（BC）溶液涂抹，苯扎氯铵是一种阳离子表面活性剂，已证明其会对哺乳动物神经元造成不可逆损伤，但不会损伤肌肉组织。2. 在进行2周的胃动力荧光观察后，对火鸡实施安乐死，切除整个肌胃并称重，同时取TCM的系列冰冻切片，以评估肌间神经丛中神经元的数量和大小。3. 与用生理盐水处理的对照组相比，用0.5%和1.0% BC处理导致TCM失去动力，CTM显著肥大（P小于0.001），肌间神经元数量减少70%，大小减小60%，浆膜厚度增加4倍。