两种淡水鱼——雅罗鱼（Chondrostoma nasus）和拟鲤（Rutilus rutilus）肝脏中某些单加氧酶活性的表征及细胞色素P - 450的增溶作用

Characterization of some monooxygenase activities and solubilization of hepatic cytochrome P-450 in two species of freshwater fish, the nase (Chondrostoma nasus) and the roach (Rutilus rutilus).

Monod G, Devaux A, Rivière J L

Institut National de la Recherche Agronomique, Laboratoire d'Ecotoxicologie INRA-ENVL, Charbonnières, France.

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1987;88(1):83-9. doi: 10.1016/0742-8413(87)90050-8.

Abstract

Hepatic monooxygenase activities were studied in microsomal fractions from two species of freshwater fish, the nase (Chondrostoma nasus) and the European roach (Rutilus rutilus). 2. These activities were determined by using four substrates, 7-ethoxycoumarin, 7-ethoxyresorufin, benzo(a)pyrene, and 2,5-diphenyloxazole and were characterized according to pH, temperature, cofactors, and the differential effects of two inhibitors, metyrapone and alpha-naphthoflavone. 3. Solubilization of microsomes was achieved by the use of detergents, with a good recovery of the cytochrome P-450.

摘要

研究了两种淡水鱼——雅罗鱼（Chondrostoma nasus）和拟鲤（Rutilus rutilus）微粒体部分的肝单加氧酶活性。2. 通过使用四种底物（7-乙氧基香豆素、7-乙氧基试卤灵、苯并(a)芘和2,5-二苯基恶唑）测定这些活性，并根据pH、温度、辅因子以及两种抑制剂（甲吡酮和α-萘黄酮）的不同作用进行表征。3. 通过使用去污剂实现微粒体的增溶，细胞色素P-450回收率良好。