Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes, 1524, Sao Paulo, SP, 05508-000, Brazil.
Cell Biol Int. 2018 Jan;42(1):95-104. doi: 10.1002/cbin.10872. Epub 2017 Oct 9.
Clinical data published in recent years have demonstrated positive effects of collagen hydrolysate (CH) on skin aging clinical signs. CH use as food supplement has a long history; however, few studies have addressed the underlying purpose of CH on the cellular and molecular biology of skin cells that could elucidate clinical improvement findings. Wide diversity of characteristics has been reported for dermal fibroblasts derived from different body sites and it is unknown whether collagen peptides could modulate differently cells from chronological aged and photoaged skin areas. This study investigated the influence of CH on the extracellular matrix metabolism and proliferation of human dermal fibroblasts (HDFs) derived from chronological aged (sun-protected) and photoaged (sun-exposed) body sites. CH treatment did not affect cellular proliferation of either cell cultures, but notably modulated cell metabolism in monolayer model, increasing the content of dermal matrix precursor and main protein, procollagen I and collagen I, respectively. These effects were confirmed in the human dermal equivalent model. The increase in collagen content in the cultures was attributed to stimulation of biosynthesis and decreased collagen I metabolism through inhibition of metalloproteinase activity (MMP) 1 and 2. Modulation of CH in dermal metabolism did not differ between cells derived from sun-protected and sun-exposed areas, although lower concentrations of CH seemed to be enough to stimulate sun-exposed-derived HDFs, suggesting more pronounced effect in these cells. This study contributes to understanding the biological effects of CH on skin cells and viability of its use as a functional ingredient in food supplements.
近年来的临床数据表明，胶原蛋白水解物（CH）对皮肤衰老的临床症状有积极影响。CH 作为食品补充剂的使用历史悠久；然而，很少有研究探讨 CH 对皮肤细胞的细胞和分子生物学的潜在作用，而这可能阐明临床改善的发现。从不同身体部位获得的真皮成纤维细胞具有广泛的特征多样性，目前尚不清楚胶原蛋白肽是否可以调节来自正常老化和光老化皮肤区域的细胞。本研究调查了 CH 对源自正常老化（防晒）和光老化（暴露于阳光）身体部位的人真皮成纤维细胞（HDF）的细胞外基质代谢和增殖的影响。CH 处理不会影响两种细胞培养物的细胞增殖，但值得注意的是，它在单层模型中调节细胞代谢，分别增加真皮基质前体和主要蛋白原胶原蛋白 I 和胶原蛋白 I 的含量。这些作用在人真皮等效模型中得到了证实。培养物中胶原蛋白含量的增加归因于刺激生物合成和通过抑制金属蛋白酶活性 (MMP) 1 和 2 来降低胶原蛋白 I 代谢。源自防晒和暴露于阳光部位的细胞之间 CH 对真皮代谢的调节没有差异，尽管较低浓度的 CH 似乎足以刺激暴露于阳光部位的 HDF，表明这些细胞的作用更为明显。这项研究有助于了解 CH 对皮肤细胞的生物学影响及其作为功能性食品补充剂成分的应用的可行性。
