Lie W R, Rothschild M F, Warner C M
Department of Biochemistry, Iowa State University, Ames 50011.
J Immunol. 1987 Nov 15;139(10):3388-95.
Three miniature swine lines, inbred for swine leukocyte antigen (SLA) haplotypes, a, c, and d, and a recombinant line, haplotype g, were analyzed for possible restriction fragment length polymorphisms (RFLP) by Southern blot hybridization with human C2, factor B (Bf), and C4 specific probes. The search for RFLP by using a human C2 probe failed to reveal any variants. However, a Taq I polymorphism was identified with the human Bf probe and Bam HI and Pvu II polymorphisms were identified with the human C4 probe. Overlapping restriction fragments were found with the C2 and Bf probes, which strongly suggests close linkage of C2 and Bf genes in swine. Segregation analyses of the Bf and C4 polymorphisms indicated that the polymorphic fragments followed a Mendelian pattern of inheritance. The recombinant haplotype g, which expresses class I genes of haplotype c and class II genes of haplotype d, was shown to produce an identical RFLP pattern, by using the Bf and C4 probes, as haplotype d, but different from that of haplotype c. This indicates that there is a close association of [C4-Bf-C2] and class II genes in miniature swine. Although these data do not show conclusively the location of the [C4-Bf-C2] genes, it is hypothesized that swine [C4-Bf-C2] genes are located between the class II and class I genes, as has been demonstrated in mouse and man.
对三个猪白细胞抗原（SLA）单倍型a、c和d的近交小型猪品系以及一个重组单倍型g的小型猪品系，使用人C2、B因子（Bf）和C4特异性探针进行Southern印迹杂交，分析其可能存在的限制性片段长度多态性（RFLP）。使用人C2探针寻找RFLP未发现任何变异。然而，使用人Bf探针鉴定出一个Taq I多态性，使用人C4探针鉴定出Bam HI和Pvu II多态性。发现C2和Bf探针存在重叠的限制性片段，这强烈提示猪中C2和Bf基因紧密连锁。对Bf和C4多态性的分离分析表明，多态性片段遵循孟德尔遗传模式。重组单倍型g表达单倍型c的I类基因和单倍型d的II类基因，使用Bf和C4探针显示其产生与单倍型d相同的RFLP模式，但与单倍型c不同。这表明小型猪中[C4 - Bf - C2]与II类基因存在紧密关联。尽管这些数据没有确凿显示[C4 - Bf - C2]基因的位置，但据推测猪的[C4 - Bf - C2]基因位于II类基因和I类基因之间，正如在小鼠和人类中所证实的那样。
