一种用于测量神经元组织中乙酰胆碱和胆碱的具有灵敏度和稳定性的高效液相色谱分析方法。
An HPLC assay procedure of sensitivity and stability for measurement of acetylcholine and choline in neuronal tissue.
作者信息
Barnes N M, Costall B, Fell A F, Naylor R J
机构信息
Pharmaceutical Analysis Research Unit, Postgraduate School of Studies in Pharmaceutical Chemistry, University of Bradford, UK.
出版信息
J Pharm Pharmacol. 1987 Sep;39(9):727-31. doi: 10.1111/j.2042-7158.1987.tb06977.x.
A method is presented for the sensitive and specific determination of acetylcholine and choline in neuronal tissue. The method is based on the separation of acetylcholine and choline by reversed-phase HPLC, passing the eluent into a post-column reactor containing choline oxidase and acetylcholinesterase covalently bound to vinyl sulphone bonded onto a hydroxyethyl methacrylate support, and electrochemical detection of the hydrogen peroxide formed. The limit of detection of the procedure is 1 pmol for acetylcholine and 500 fmol for choline. The excellent baseline stability of the method ensures the rapid and reliable processing of a large number of samples.
本文介绍了一种用于灵敏且特异测定神经元组织中乙酰胆碱和胆碱的方法。该方法基于反相高效液相色谱法分离乙酰胆碱和胆碱，将洗脱液通入含有共价结合到乙烯基砜上并固定在甲基丙烯酸羟乙酯载体上的胆碱氧化酶和乙酰胆碱酯酶的柱后反应器中，然后对生成的过氧化氢进行电化学检测。该方法对乙酰胆碱的检测限为1皮摩尔，对胆碱的检测限为500飞摩尔。该方法出色的基线稳定性确保了大量样品能够快速且可靠地处理。
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