Renold F K, Dazin P, Goetzl E J, Payan D G
Howard Hughes Medical Institute, University of California Medical Center, San Francisco.
J Neurosci Res. 1987;18(1):195-202. doi: 10.1002/jnr.490180128.
Receptors for somatostatin (SOM) were identified on mouse bone marrow derived mast cells (MBMMC) and shown to vary in expression with the state of proliferation and differentiation of the MBMMC. Flow cytometric studies of the binding of fluorescein-labeled SOM and concurrent analyses of the binding of [125I]SOM demonstrated that the population of MBMMC capable of recognizing SOM specifically is that exhibiting a proliferative response to interleukin-3. The MBMMC that bound SOM reached a maximal number at 72 hr following the addition of interleukin-3, and were distributed principally in the G2/M phase of the cell cycle. SOM did not influence directly the proliferative responses of MBMMC to interleukin-3. The level of expression of SOM receptors thus may reflect the state of differentiation of mast cells, as well as determining the functional sensitivity to the inhibitory effects of SOM.
在小鼠骨髓来源的肥大细胞（MBMMC）上鉴定出了生长抑素（SOM）受体，并且发现其表达会随着MBMMC的增殖和分化状态而变化。对荧光素标记的SOM结合进行的流式细胞术研究以及对[125I]SOM结合的同步分析表明，能够特异性识别SOM的MBMMC群体是那些对白介素-3表现出增殖反应的细胞。在添加白介素-3后72小时，结合SOM的MBMMC数量达到最大值，并且主要分布在细胞周期的G2/M期。SOM并不直接影响MBMMC对白介素-3的增殖反应。因此，SOM受体的表达水平可能反映肥大细胞的分化状态，同时也决定了对SOM抑制作用的功能敏感性。
