Sumedha S, Kotrashetti V S, Nayak R S, Nayak A, Raikar A
a Departments of Oral Pathology and Microbiology.
b Periodontology , Maratha Mandal's NG Halgekar Institute of Dental Sciences and Research Centre , Belgaum , Karnataka , India.
Biotech Histochem. 2017;92(7):487-497. doi: 10.1080/10520295.2017.1357192. Epub 2017 Sep 14.
We used immunohistochemistry to quantify and compare the expression of Toll-like receptor 2 (TLR2) and cluster of differentiation 14 (CD14) in gingival tissues of both healthy individuals and patients with chronic periodontitis. We also correlated the expression of TLR2 and CD14 with the histological grades of chronic periodontitis. We examined 30 gingival specimens from chronic periodontitis patients and 10 from healthy individuals. Tissues from both groups were immunostained with antibodies against TLR2 and CD14. TLR2 and CD14 were expressed by endothelial cells, fibroblasts, lymphocytes and plasma cells. The immunohistochemical expression of TLR2 and CD14 was significantly greater in inflammatory cells of the chronic periodontitis group than in healthy individuals. Expression of these molecules was greater in the inflammatory cells of connective tissue adjacent to pocket epithelium in both groups. The expression of TLR2 and CD14 was greatest in the periodontitis group that was classified as severe grade, followed by moderate and mild grades, which suggests a role of TLR2 and CD14 in the pathogenesis of chronic periodontitis. The positive correlation of TLR2 and CD14 expression levels with the severity grades of chronic periodontitis suggests that they are correlated also with disease severity; therefore, they may be useful for predicting disease progression. Our findings are consistent with the possibility that CD14 acts as a co-receptor for TLR2.
我们采用免疫组织化学方法对健康个体和慢性牙周炎患者牙龈组织中Toll样受体2（TLR2）和分化簇14（CD14）的表达进行定量和比较。我们还将TLR2和CD14的表达与慢性牙周炎的组织学分级相关联。我们检查了30例慢性牙周炎患者的牙龈标本和10例健康个体的牙龈标本。两组组织均用抗TLR2和CD14抗体进行免疫染色。TLR2和CD14在内皮细胞、成纤维细胞、淋巴细胞和浆细胞中表达。慢性牙周炎组炎症细胞中TLR2和CD14的免疫组化表达明显高于健康个体。两组中与袋上皮相邻的结缔组织炎症细胞中这些分子的表达更高。TLR2和CD14在重度慢性牙周炎组中的表达最高，其次是中度和轻度组，这表明TLR2和CD14在慢性牙周炎发病机制中起作用。TLR2和CD14表达水平与慢性牙周炎严重程度分级呈正相关，表明它们也与疾病严重程度相关；因此，它们可能有助于预测疾病进展。我们的研究结果与CD14作为TLR2共受体的可能性一致。
