Murakami Akira, Nesumi Atsushi, Maeda-Yamamoto Mari, Yamaguchi Hidekazu, Yashima Koji, Miura Masahiro, Nakano Tsuyoshi, Nekoshima Kazunori
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
National Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization, Setocho, Makurazaki, Kagoshima 898-0087, Japan.
J Food Drug Anal. 2015 Sep;23(3):407-416. doi: 10.1016/j.jfda.2014.11.004. Epub 2015 Feb 16.
Sunrouge is an anthocyanin-rich, new tea cultivar that contains similar levels of catechins as Yabukita, the most popular tea cultivar consumed in Japan. Interestingly, Sunrouge preparations have previously been shown to have more pronounced acetylcholinesterase inhibitory and anticolitis activities than those of Yabukita. In this study, we examined their effects on expressions of self-defensive molecules, including heat shock proteins (HSPs), which are molecular chaperones involved in homeostasis and longevity. Hot water extract from freeze-dried Sunrouge significantly upregulated messenger RNA (mRNA) expressions of HSP40, HSP70, and HSP32 (heme oxygenase-1), with grades greater than those shown by Yabukita. Oral administration of freeze-dried preparation of Sunrouge to male ICR mice at a dose of 1% in the basal diet for 1 month resulted in marked upregulations of several HSP mRNA expressions in mucosa from the gastrointestinal tract, especially the upper small intestine. Again, its efficacy was remarkably higher than that of Yabukita. Moreover, exposure of Caenorhabditis elegans to Sunrouge conferred thermoresistant phenotype, and also resulted in a significant life-span elongation. Taken together, our results suggest that Sunrouge is a unique and promising tea cultivar for regulating self-defense systems.
红玉是一种富含花青素的新型茶树品种，其儿茶素含量与日本最受欢迎的茶树品种薮北相当。有趣的是，此前研究表明，红玉制剂比薮北制剂具有更显著的乙酰胆碱酯酶抑制活性和抗结肠炎活性。在本研究中，我们检测了它们对包括热休克蛋白（HSPs）在内的自我防御分子表达的影响，热休克蛋白是参与体内稳态和长寿的分子伴侣。冻干红玉的热水提取物显著上调了HSP40、HSP70和HSP32（血红素加氧酶-1）的信使核糖核酸（mRNA）表达，上调程度高于薮北。以1%的剂量将冻干红玉制剂添加到基础饮食中，对雄性ICR小鼠进行为期1个月的口服给药，结果导致胃肠道尤其是十二指肠黏膜中几种HSP mRNA表达显著上调。同样，其效果明显高于薮北。此外，秀丽隐杆线虫接触红玉后呈现耐热表型，并且寿命显著延长。综上所述，我们的结果表明，红玉是一种独特且有前景的用于调节自我防御系统的茶树品种。
