Young S H, Chow I
Department of Physiology, UCLA School of Medicine 90024.
Science. 1987 Dec 18;238(4834):1712-3. doi: 10.1126/science.2891190.
The traditional view that quantal release of neurotransmitter results from the fusion of transmitter-containing vesicles with the neuronal membrane has been recently challenged. Although various alternative mechanisms have been proposed, a common element among them is the release of cytoplasmic transmitter, which, in one view, could occur through large conductance channels on the presynaptic membrane. Six nerve-muscle cell pairs were examined with a whole-cell patch clamp for the presence of such channels that are associated with the production of miniature end-plate potentials. Examination of the neuronal membrane current during the occurrence of 822 miniature end-plate potentials produced no evidence of large channels. Thus it is unlikely that quantal release is mediated by such channels in the neuromuscular junction.
神经递质的量子释放是由含递质的囊泡与神经元膜融合所致，这一传统观点最近受到了挑战。尽管已经提出了各种替代机制，但它们的一个共同要素是细胞质递质的释放，从一种观点来看，这可能通过突触前膜上的大电导通道发生。用全细胞膜片钳检查了六对神经肌肉细胞，以寻找与微小终板电位产生相关的此类通道。在822个微小终板电位出现期间对神经元膜电流进行检测，未发现大通道存在的证据。因此，量子释放在神经肌肉接头处不太可能由此类通道介导。
