Salsano Stefania, Quiñonero Alicia, Pérez Silvia, Garrido Gómez Tamara, Simón Carlos, Dominguez Francisco
Fundación Instituto Valenciano de Infertilidad (FIVI), Instituto Universitario IVI (IUIVI), Valencia, Spain.
Fundación Instituto Valenciano de Infertilidad (FIVI), Instituto Universitario IVI (IUIVI), Valencia, Spain; Igenomix Academy, Valencia, Spain; Department of Pediatrics, Obstetrics and Gynaecology, School of Medicine, Valencia University, Valencia, Spain.
Fertil Steril. 2017 Nov;108(5):832-842.e1. doi: 10.1016/j.fertnstert.2017.07.1163. Epub 2017 Sep 11.
To characterize PGRMC1 and SERBP1 in human endometrium and to investigate the putative role of PGRMC1 in endometrial decidualization.
The PGRMC1 and SERBP1 expression in human endometrium was determined throughout the menstrual cycle. We analyzed the colocalization of PGRMC1 and SERBP1. Then, endometrial stromal cells (ESCs) were isolated to investigate the functional effect of PGRMC1 overexpression on decidualization.
IVI clinic.
PATIENT(S): Endometrial biopsies were collected from fertile volunteers (n = 61) attending the clinic as ovum donors.
INTERVENTION(S): Endometrial samples of 61 healthy fertile women.
MAIN OUTCOME MEASURE(S): In vivo localization of PGRMC1 and SERBP1 was assessed by immunohistochemistry. The PGRMC1/SERBP1 colocalization was investigated in vitro and in vivo. Decidualization effect of PGRMC1 overexpression was evaluated in primary ESC cultures.
RESULT(S): The PGRMC1 was detected in the endometrial stroma throughout the menstrual cycle, but decreased in the late secretory phase. The SERBP1 immunostaining was present in stroma and increased in the entire the menstrual cycle. The PGRMC1 and SERBP1 colocalized in the cytoplasmic fractions of nondecidualized and decidualized ESC. The PGRMC1 overexpression significantly inhibited in vitro decidualization.
CONCLUSION(S): Our results suggest that classic P receptors (PRs) are not the only kind playing a role in the normal physiology of the endometrium. The human decidualization process could be altered by the overexpression or mislocalization of PGRMC1 in ESC.
鉴定人子宫内膜中的孕酮受体膜组分1（PGRMC1）和丝氨酸/精氨酸丰富剪接因子结合蛋白1（SERBP1），并研究PGRMC1在子宫内膜蜕膜化中的潜在作用。
测定人子宫内膜在整个月经周期中PGRMC1和SERBP1的表达。分析PGRMC1和SERBP1的共定位情况。然后，分离子宫内膜基质细胞（ESC），以研究PGRMC1过表达对蜕膜化的功能影响。
IVI诊所。
从作为卵子捐赠者前往该诊所的有生育能力的志愿者（n = 61）中收集子宫内膜活检样本。
61名健康有生育能力女性的子宫内膜样本。
通过免疫组织化学评估PGRMC1和SERBP1在体内的定位。在体外和体内研究PGRMC1/SERBP1的共定位。在原代ESC培养物中评估PGRMC1过表达的蜕膜化作用。
在整个月经周期的子宫内膜基质中均检测到PGRMC1，但在分泌晚期减少。SERBP1免疫染色存在于基质中，且在整个月经周期中增加。PGRMC1和SERBP1在未蜕膜化和蜕膜化的ESC的细胞质部分中共定位。PGRMC1过表达显著抑制体外蜕膜化。
我们的结果表明，经典孕酮受体（PRs）并非在子宫内膜正常生理过程中发挥作用的唯一类型受体。ESC中PGRMC1的过表达或定位错误可能会改变人蜕膜化过程。
Zotero 插件