Meakins-Christie Laboratories and the Department of Human Genetics, McGill University Montreal, PQ, Canada.
Meakins-Christie Laboratories and the Department of Medicine, McGill University, Montreal, PQ, Canada.
Sci Rep. 2017 Sep 14;7(1):11586. doi: 10.1038/s41598-017-11656-5.
Radiotherapy can result in lung diseases pneumonitis or fibrosis dependent on patient susceptibility. Herein we used inbred and genetically altered mice to investigate whether the tissue adaptive immune response to radiation injury influences the development of radiation-induced lung disease. Six inbred mouse strains were exposed to 18 Gy whole thorax irradiation and upon respiratory distress strains prone to pneumonitis with fibrosis presented an increased pulmonary frequency of Thelper (Th)17 cells which was not evident in strains prone solely to pneumonitis. The contribution of Th17 cells to fibrosis development was supported as the known enhanced fibrosis of toll-like receptor 2&4 deficient mice, compared to C57BL/6J mice, occurred with earlier onset neutrophilia, and with increased levels of pulmonary Th17, but not Th1, cells following irradiation. Irradiated Il17-/- mice lacked Th17 cells, and were spared both fibrosis and pneumonitis, as they survived to the end of the experiment with a significantly increased pulmonary Th1 cell frequency, only. Interferon-γ-/- mice, deficient in Th1 cells, developed a significantly enhanced fibrosis response compared to that of C57BL/6J mice. The tissue adaptive immune response influences the pulmonary disease response to radiotherapy, as an increased Th17 cell frequency enhanced and a Th1 response spared, fibrosis in mice.
放射疗法可导致肺炎或肺纤维化等肺部疾病,具体取决于患者的易感性。在此,我们使用近交系和基因修饰小鼠来研究组织适应性免疫反应对辐射损伤的影响是否会影响放射性肺损伤的发展。将六种近交系小鼠暴露于 18Gy 全胸照射,在出现呼吸窘迫的情况下,易发生肺炎伴纤维化的小鼠肺中 Th17 细胞的频率增加,而仅易发生肺炎的小鼠则不明显。Th17 细胞对纤维化发展的贡献得到支持,因为与 C57BL/6J 小鼠相比, Toll 样受体 2&4 缺陷小鼠的纤维化增强,其中性粒细胞增多更早出现,并且在照射后肺 Th17 细胞水平升高,而 Th1 细胞水平不变。照射后的 Il17-/- 小鼠缺乏 Th17 细胞,既没有纤维化也没有肺炎,因为它们在实验结束时存活下来,肺 Th1 细胞频率显著增加。缺乏 Th1 细胞的干扰素-γ-/- 小鼠与 C57BL/6J 小鼠相比,纤维化反应明显增强。组织适应性免疫反应影响放射治疗后的肺部疾病反应,因为 Th17 细胞频率增加和 Th1 反应减轻,可使小鼠发生纤维化。