慢性非结核分枝杆菌感染患者肺部免疫细胞的特征分析
Characterization of Immune Cells From the Lungs of Patients With Chronic Non-Tuberculous Mycobacteria or Infection.
作者信息
Schenkel Alan R, Mitchell John D, Cool Carlyne D, Bai Xiyuan, Groshong Steve, Koelsch Tilman, Verma Deepshikha, Ordway Diane, Chan Edward D
机构信息
Department of Microbiology, Immunology, & Pathology, Colorado State University, Fort Collins, CO 80523, USA.
Division of Cardiothoracic Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
出版信息
Immune Netw. 2022 Apr 11;22(3):e27. doi: 10.4110/in.2022.22.e27. eCollection 2022 Jun.
Little is known of the lung cellular immunophenotypes in patients with non-tuberculous mycobacterial lung disease (NTM-LD). Flow-cytometric analyses for the major myeloid and lymphoid cell subsets were performed in less- and more-diseased areas of surgically resected lungs from six patients with NTM-LD and two with lung disease (-LD). Lymphocytes, comprised mainly of NK cells, CD4 and CD8 T cells, and B cells, accounted for ~60% of all leukocytes, with greater prevalence of T and B cells in more-diseased areas. In contrast, fewer neutrophils were found with decreased number in more-diseased areas. Compared to NTM-LD, lung tissues from patients with -LD demonstrated relatively lower numbers of T and B lymphocytes but similar numbers of NK cells. While this study demonstrated a large influx of lymphocytes into the lungs of patients with chronic NTM-LD, further analyses of their phenotypes are necessary to determine the significance of these findings.
关于非结核分枝杆菌肺病（NTM-LD）患者的肺细胞免疫表型，人们所知甚少。对6例NTM-LD患者和2例肺病（-LD）患者手术切除肺组织中病变较轻和较重区域的主要髓系和淋巴细胞亚群进行了流式细胞术分析。淋巴细胞主要由自然杀伤（NK）细胞、CD4和CD8 T细胞以及B细胞组成，占所有白细胞的约60%，在病变较重区域T细胞和B细胞的比例更高。相比之下，中性粒细胞数量较少，且在病变较重区域数量减少。与NTM-LD患者相比，-LD患者的肺组织中T淋巴细胞和B淋巴细胞数量相对较少，但NK细胞数量相似。虽然这项研究表明慢性NTM-LD患者的肺部有大量淋巴细胞浸润，但有必要对其表型进行进一步分析，以确定这些发现的意义。
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