Murray C J
Salmonella Reference Laboratory, Institute of Medical and Veterinary Science, Adelaide, South Australia.
Aust Vet J. 1987 Aug;64(8):239-40. doi: 10.1111/j.1751-0813.1987.tb09691.x.
Coagglutination was used to detect K88 and K99 fimbrial antigens on Escherichia coli, and results were compared to an enzyme immuno assay (EIA). When pili suspensions were tested by both methods, 28 of 66 cultures were shown to have K88 and 11 of 31 cultures had K99 antigens. No pili suspensions were positive by coagglutination that were not positive by EIA. Testing of cell suspensions gave equivalent results to pili suspensions for K99 when tested by coagglutination. Two cell suspensions reacted with the K88 coagglutination which could not be confirmed by testing of pili suspensions, while a further 20 out of 43 cultures gave equivalent results with both cell and pili suspensions for K88 when tested by coagglutination.
采用协同凝集试验检测大肠杆菌上的K88和K99菌毛抗原，并将结果与酶免疫测定（EIA）进行比较。当用两种方法检测菌毛悬液时，66份培养物中有28份显示有K88抗原，31份培养物中有11份有K99抗原。协同凝集试验呈阳性的菌毛悬液，酶免疫测定也均为阳性。用协同凝集试验检测细胞悬液时，对于K99抗原，其结果与菌毛悬液相当。有两份细胞悬液与K88协同凝集反应呈阳性，但菌毛悬液检测无法证实，而在43份培养物中，另有20份培养物在用协同凝集试验检测K88抗原时，细胞悬液和菌毛悬液的结果相当。
