Bhalla Prem L, Sharma Akanksha, Singh Mohan B
Plant Molecular Biology and Biotechnology Laboratory, School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia.
Methods Mol Biol. 2017;1679:3-24. doi: 10.1007/978-1-4939-7337-8_1.
Wheat is the major staple food crop and a source of calories for humans worldwide. A steady increase in the wheat production is essential to meet the demands of an ever-increasing global population and to achieve food security. The large size and structurally intricate genome of polyploid wheat had hindered the genomic analysis. However, with the advent of new genomic technologies such as next generation sequencing has led to genome drafts for bread wheat and its progenitors and has paved the way to design new strategies for crop improvement. Here we provide an overview of the advancements made in wheat genomics together with the available "omics approaches" and bioinformatics resources developed for wheat research. Advances in genomic, transcriptomic, and metabolomic technologies are highlighted as options to circumvent existing bottlenecks in the phenotypic and genomic selection and gene transfer. The contemporary reverse genetics approaches, including the novel genome editing techniques to inform targeted manipulation of a single/multiple genes and strategies for generating marker-free transgenic wheat plants, emphasize potential to revolutionize wheat improvement shortly.
小麦是主要的主食作物,也是全球人类热量的来源。小麦产量的稳步增长对于满足不断增长的全球人口需求以及实现粮食安全至关重要。多倍体小麦庞大且结构复杂的基因组阻碍了基因组分析。然而,随着新一代测序等新基因组技术的出现,已产生了面包小麦及其祖先的基因组草图,并为设计作物改良新策略铺平了道路。在此,我们概述了小麦基因组学取得的进展,以及为小麦研究开发的可用“组学方法”和生物信息学资源。基因组学、转录组学和代谢组学技术的进展被重点介绍,它们是规避表型和基因组选择以及基因转移中现有瓶颈的选择。当代反向遗传学方法,包括用于指导单个/多个基因靶向操作的新型基因组编辑技术以及生成无标记转基因小麦植株的策略,强调了在不久的将来彻底改变小麦改良的潜力。