Thakkar Prachi Jayesh, Badakar Chandrashekhar M, Hugar Shivayogi M, Hallikerimath Seema, Patel Punit M, Shah Parin
Department of Pedodontics and Preventive Dentistry, KLE VK Institute of Dental Sciences, Belagavi, Karnataka, India.
Department of Oral Pathology and Microbiology, KLE VK Institute of Dental Sciences, Belagavi, Karnataka, India.
J Indian Soc Pedod Prev Dent. 2017 Oct-Dec;35(4):312-318. doi: 10.4103/JISPPD.JISPPD_308_16.
This study aims to determine and compare the extent of inhibition of demineralization and promotion of remineralization of permanent molar enamel with and without application of three remineralizing agents.
Forty extracted permanent molars were randomly divided into two groups 1 and 2, longitudinally sectioned into four and divided into subgroups A, B, C, and D. The sections were coated with nail varnish leaving a window of 3 mm × 3 mm. All sections of Group 1 were treated with their respective subgroup-specific agent: Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste for subgroup A, CPP-amorphous calcium phosphate fluoride (ACPF) paste for subgroup B, CPP-ACPF varnish for subgroup C and subgroup D served as a control. The sections were then subjected to demineralization for 12 days following which lesional depth was measured under the stereomicroscope. All the sections of Group 2 were subjected to demineralization for 12 days, examined for lesional depth, then treated with their respective subgroup specific agents and immersed in artificial saliva for 7 days. The sections were then examined again under the stereomicroscope to measure the lesional depth.
CPP-ACPF varnish caused significant inhibition of demineralization. All three agents showed significant remineralization of previously demineralized lesions. However, CPP-ACPF varnish showed the greatest remineralization, followed by CPP-ACPF paste and then CPP-ACP paste.
This study shows that CPP-ACPF varnish is effective in preventing demineralization as well as promoting remineralization of enamel. Thus, it can be used as an effective preventive measure for pediatric patients where compliance with the use of tooth mousse may be questionable.
本研究旨在确定并比较在应用和未应用三种再矿化剂的情况下，恒牙磨牙牙釉质脱矿抑制和再矿化促进的程度。
40颗拔除的恒牙磨牙随机分为1组和2组，纵向切成四份并分为A、B、C和D亚组。各切片用指甲油涂抹，留出3mm×3mm的窗口。第1组的所有切片用各自亚组特异性试剂处理：A亚组用酪蛋白磷酸肽 - 无定形磷酸钙（CPP - ACP）糊剂，B亚组用CPP - 无定形磷酸钙氟化物（ACPF）糊剂，C亚组用CPP - ACPF清漆，D亚组作为对照。然后将切片进行12天的脱矿处理，之后在体视显微镜下测量病损深度。第2组的所有切片进行12天的脱矿处理，检查病损深度，然后用各自亚组特异性试剂处理，并浸入人工唾液中7天。然后再次在体视显微镜下检查切片以测量病损深度。
CPP - ACPF清漆对脱矿有显著抑制作用。所有三种试剂对先前脱矿的病损均显示出显著的再矿化作用。然而，CPP - ACPF清漆的再矿化作用最强，其次是CPP - ACPF糊剂，然后是CPP - ACP糊剂。
本研究表明，CPP - ACPF清漆在预防牙釉质脱矿以及促进其再矿化方面是有效的。因此，它可作为一种有效的预防措施用于儿科患者，在这些患者中使用护齿泡沫的依从性可能存在问题。
